A5037626063- Bacterial biofilms and quorum sensing
- Bacterial Genetics and Biotechnology
- Biochemical and biochemical processes
- Hemoglobin structure and function
- Biotin and Related Studies
- Iron Metabolism and Disorders
- Antibiotic Resistance in Bacteria
- Metal-Catalyzed Oxygenation Mechanisms
- Porphyrin Metabolism and Disorders
- Vibrio bacteria research studies
- Enzyme Structure and Function
- Heme Oxygenase-1 and Carbon Monoxide
- Legionella and Acanthamoeba research
Princeton University
2023
Queen's University
2014-2018
The outer membrane of Gram-negative bacteria is unique in both structure and function. surface-exposed leaflet composed lipopolysaccharide, while the inner glycerophospholipids. This lipid asymmetry creates mechanical strength, lowers permeability, necessary for virulence many pathogens. Glycerophospholipids that mislocalize to are removed by Mla pathway, which consists channel MlaA, periplasmic carrier MlaC, transporter MlaBDEF. opportunistic pathogen
Most bacteria possess only one heme-degrading enzyme for obtaining iron, however few such as Pseudomonas aeruginosa express two, namely PhuS and HemO. While HemO is a well-known heme oxygenase, previously we discovered that also possesses degradation activity generates verdoheme, an intermediate of breakdown. To understand the coexistence these two enzymes, using DFT calculation reveal effectively enhances through its participation in hydroxylation, rate limiting reaction. Heme converted to...
Protein crystallization is the major bottleneck in entire process of protein crystallography, and obtaining diffraction-quality crystals can be unpredictable sometimes exceptionally difficult, requiring many rounds high-throughput screening. Recently, a more time- cost-saving strategy to use commercially available microfluidic devices called Crystal Formers has emerged. Herein we show application such device using from Legionella pneumophila LidL that predicted involved ability efficiently...