A5016314781- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- vaccines and immunoinformatics approaches
- Complement system in diseases
- Virus-based gene therapy research
- Immune Cell Function and Interaction
- Cancer Research and Treatments
BioMed X Institute
2023-2024
Effective, unbiased, high-throughput methods to functionally identify both class II and I HLA-presented T cell epitopes their cognate receptors (TCRs) are essential for prerequisite diagnostic therapeutic applications, yet remain underdeveloped. Here, we present T-FINDER [T Functional Identification (Neo)-antigen Discovery of Epitopes Receptors], a system rapidly deconvolute CD4 CD8 TCRs targets physiologically processed presented by an individual's unmanipulated, complete human leukocyte...
H3K27M, a driver mutation with T and B cell neoepitope characteristics, defines an aggressive subtype of diffuse glioma poor survival. We functionally dissect the immune response one patient treated H3K27M peptide vaccine who subsequently entered complete remission. The robustly expanded class II human leukocyte antigen (HLA)–restricted peripheral H3K27M-specific cells. Using functional assays, we characterized 34 clonally unique H3K27M-reactive receptors identified critical, conserved...
Abstract H3K27M, a driver mutation with T- and B-cell neoepitope characteristics, defines an aggressive subtype of diffuse glioma poor survival. We functionally dissect the immune response one patient who was treated H3K27M peptide vaccine subsequently entered complete remission. The robustly expanded class II HLA-restricted peripheral H3K27M-specific T cells. Using functional assays, we characterized 34 clonally unique H3K27M-reactive cell receptors identified critical, conserved motifs in...
Abstract Effective, unbiased, high-throughput methods to functionally identify both class II and I HLA-presented T cell epitopes their cognate receptors (TCRs) are essential for prerequisite diagnostic therapeutic applications, yet remain underdeveloped. Addressing this bottleneck, we established T-FINDER (T Functional Identification (Neo)-antigen Discovery of Epitopes Receptors), a platform that rapidly deconvolutes CD4 CD8 TCR reactivities targets physiologically processed presented by an...
Abstract Antigen processing and presentation are crucial for T-cell receptor engagement potent adaptive immune responses. While establishing an antigen system to identify TCR-epitope pairs from large libraries of genetically templated, ectopically expressed antigens, we observed a longitudinal suppression in B cells, which call attenuation (APA). Beyond its potential role regulating cell-enforced peripheral tolerance, APA represents bottleneck sustained screening vaccination approaches. To...
<h3>Background</h3> Previously, we demonstrated that 5/3 chimeric oncolytic adenoviruses coding for human mucin1 (MUC1) T cell engager boosted intratumoral activation and proliferation, resulting in effective antitumor efficacy A549 patient-derived xenograft (PDX) ovarian cancer mouse models. In this study, constructed characterized novel adenovirus, Ad5/3-E2F-d24-aMUC1aCD3-IRES-IL2 (TILT-322) built on a backbone of Ad5/3-E2F-d24 carrying MUC1 transgene. Additionally, interleukin (IL)-2,...