A5077984414- Extracellular vesicles in disease
- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Sulfur-Based Synthesis Techniques
- Cannabis and Cannabinoid Research
- Nanoplatforms for cancer theranostics
- Catalytic C–H Functionalization Methods
- Analytical Chemistry and Chromatography
- MicroRNA in disease regulation
- Catalytic Alkyne Reactions
- SARS-CoV-2 detection and testing
- Forensic Toxicology and Drug Analysis
- Microfluidic and Capillary Electrophoresis Applications
- Drilling and Well Engineering
- Advanced Nanomaterials in Catalysis
- Reservoir Engineering and Simulation Methods
- Metabolomics and Mass Spectrometry Studies
- Oil and Gas Production Techniques
- Molecular Biology Techniques and Applications
- Nanopore and Nanochannel Transport Studies
- RNA modifications and cancer
University of Ottawa
2020-2025
Weatherford (Norway)
2019
Ligand-enabled oxidative addition of Csp2-X bonds to Au(I) centers has recently appeared as a valuable strategy for the development catalytic RedOx processes. Several cross-coupling reactions that were previously considered difficult achieve reported lately, thus expanding synthetic potential gold(I) complexes beyond traditional nucleophilic functionalization π-systems. MeDalPhos played an important role in this and, despite several studies on alternative structures, remains, so far, only...
The spread of COVID-19 has affected billions people across the globe, and diagnosis viral infection still needs improvement. Because high immunogenicity abundant expression during infection, SARS-CoV-2 nucleocapsid (N) protein could be an important diagnostic marker. This study aimed to develop a label-free optical aptasensor fabricated with novel single-stranded DNA aptamer detect N protein. N-binding aptamers selected using asymmetric-emulsion PCR-SELEX their binding affinity...
Breast cancer (BC) is the second most frequently diagnosed and accounts for approximately 25% of new cases in Canadian women. Using biomarkers as a less-invasive BC diagnostic method currently under investigation but not ready practical application clinical settings. During last decade, extracellular vesicles (EVs) have emerged promising source because they contain cancer-derived proteins, RNAs, metabolites. In this study, EV proteins from small EVs (sEVs) medium (mEVs) were isolated...
A nanosheath-flow capillary electrophoresis mass spectrometry (CE-MS) system with electrospray ionization was used to profile cationic metabolite cargo in exosomes secreted by nontumorigenic MCF-10A and tumorigenic MDA-MB-231 breast epithelial cells. An in-house-produced sheath liquid interface developed machined from PEEK enable nanoflow volumes. Normalization of CE-MS peak areas the total UV signal employed enhance quantitative accuracy reduce variability. CE-MS-based metabolomics revealed...
Small membrane-derived extracellular vesicles have been proposed as participating in several cancer diseases, including breast (BC). We performed a phosphoproteomic analysis of cancer-derived small (sEVs) to provide insight into the molecular and cellular regulatory mechanisms important for tumor progression metastasis. examined three cell line models cancer: MCF10A (non-malignant), MCF7 (estrogen progesterone receptor-positive, metastatic), MDA-MB-231 (triple-negative, highly metastatic)....
DNA aptamers have many benefits for cell imaging, such as high affinity and specificity, easiness of chemical functionalization, low cost production. Among known aptamers, Sgc8-aptamer was selected against acute lymphoblastic leukemia cells with a dissociation constant in nanomolar range. The aptamer previously used the covalent coupling fluorescent magnetic nanoparticles, well fabrication aptamer-based biosensors. commonly tags, lanthanide nanoparticles offer stable luminescence narrow,...
Extracellular Vesicles (EVs) gained significant interest within the last decade as a new source of biomarkers for early detection diseases and promising tool therapeutic applications. In this work, we present Quantitative Capillary Electrophoresis (EVqCE) to measure an average mass RNA in EVs, determine EV concentrations degree degradation after sample handling. We used EVqCE analyze EVs isolated from conditioned media three cancer cell lines. employs capillary zone electrophoresis with...
Abstract The purpose of the work performed is to show possibility using geosteering not only for achieving geological targets but also supporting directional drilling operations in multilateral wells. Drilling "fishbone" design well complex terrigenous section Pokur suite Russkoye field reviewed as an example. Three kick-off with use Rotary Steerable System were carried out open hole when a horizontal under review. logs previous used optimal selection point. To control distance reservoir...
Quantification of major cannabinoids in cannabis products is normally performed using high-pressure liquid chromatography (HPLC)-based methods. We propose a cost-effective alternative method that successfully separates and quantifies 14 single run capillary electrophoresis (CE) coupled with UV detector 18 min. The separation carried out 60% acetonitrile the presence 6.5 mM sodium hydroxide 25 µM β-cyclodextrin, resulting good cannabinoids. Our CE demonstrated limit detection between 1.2–1.8...
Ligand-enabled oxidative addition of Csp2-X bonds to Au(I) centers has recently appeared as a valuable strategy for the development catalytic RedOx processes. Several cross-coupling reactions that were previously considered difficult achieve reported lately, thus expanding synthetic potential gold(I) complexes beyond traditional nucleophilic functionalization pi-systems. MeDalPhos played an important role in this and, despite several studies on alternative structures, remains so far, only...
Abstract The legalization of cannabis has magnified the importance determining quantity and identification cannabinoids. Both industry consumers are highly interested in content cannabinoids available their products, while health care professionals regulators concerned with safety cannabis. Quantification major products answers some these concerns. Currently, popular methods quantifying use high-pressure liquid chromatography (HPLC). Still, HPLC limited to a small number unless more powerful...