Patients with trisomy 21 [Down syndrome (DS)] progressively develop amyloid β-protein (Aβ) deposits and then other features of Alzheimer's disease (AD), apparently due to increased gene dosage thus expression the β-amyloid precursor protein. Because neuropathological phenotype in older DS subjects closely resembles that AD, examination brains increasing age provides a unique model progression AD. Here, we characterized deposition several Aβ peptides apolipoprotein E formalin-fixed brain...

A polymerase chain reaction (PCR) method was developed that capable of detecting a wide range medically important fungi from clinical specimens. The primer pair designed in conserved sequences 18S-ribosomal RNA genes shared by most fungi. lower limit detection this PCR technique 1 pg Candida albicans genomic DNA ethidium bromide staining and 100 fg after Southern analysis. 687-bp product amplified successfully all 78 strains 25 fungal species studies, including spp., Hansenula Saccharomyces...

Butenafine hydrochloride, N-4-tert-butylbenzyl-N-methyl-1-naphthalenemethylamine hydrochloride (butenafine), is a novel antifungal agent of the class benzylamine derivatives. was investigated for its activity against guinea pig dermatophytosis caused by Trichophyton mentagrophytes or Microsporum canis in comparison with those naftifine, tolnaftate, clotrimazole, and bifonazole. Topical butenafine showed excellent efficacy when it applied once daily, effect superior to all four reference...

Murine peritoneal macrophages were preincubated with amphotericin B (AMPH) and then stimulated bacterial lipopolysaccharide or streptococcal preparation (OK432). These produced a large amount of tumor necrosis factor. When administered to mice, the priming activity for factor production in vivo was also observed.

The stability of a two-dimensional flow in symmetric channel with suddenly expanded part is investigated numerically and analyzed by using the method nonlinear theory. From results numerical simulation, it shown that steady, unique at very low Reynolds numbers, while loses its critical number resulting an appearance asymmetric flow. transition from steady to one found occur due symmetry breaking pitchfork bifurcation when aspect ratio, ratio length width, large. It also bifurcated becomes...

AbstractSenile plaques and cerebrovascular amyloidosis are major histopathological lesions in the brains of aged dogs. Different types amyloid beta protein (Aβ) positive known: diffuse ones neuritic plaques. Diffuse may contain membrane-bound Aβ and/or small amounts fibrils. Neuritic cored with clusters fibrils degenerating neurites. In human plaques, a pathogenetic role for mcroglia cells has been described. The aim this investigation was to study microglia relationship canine investigate...

Pathogenesis of complex infection with Candida albicans and gram-negative bacteria was studied by determining the influence Escherichia coli or injection E. lipopolysaccharide (LPS) on mortality C. albicans-infected mice. Mice were infected intravenously lethal doses albicans, then treated at various times viable LPS, which individually not lethal. Treatments 3 h after clearly facilitated death Corresponding to this death, production tumor necrosis factor (TNF) in sera augmented 2 LPS into...

The mechanism of action a new benzylamine antimycotic, butenafine hydrochloride, was studied in Candida albicans by using the thiocarbamate antimycotic tolnaftate as reference drug. Butenafine completely inhibited growth test strain C. at 25 micrograms/ml and cidal 50 micrograms/ml. Tolnaftate did not show any growth-inhibitory activity up to 100 Both squalene epoxidation albicans, with 50% inhibitory concentrations being 0.57 0.17 microgram/ml, respectively. Butenafine, but tolnaftate,...

The in-vitro antifungal activities of BAY N 7133, a new orally absorbed synthetic azole derivative against 364 strains fungal pathogens were studied in direct comparison with another active compound ketoconazole, using an agar dilution procedure. 7133 was found to havea broad spectrum antifunga] activity and inhibit most pathogenic yeasts fungi at concentrations ranging from ≤0.04 10 mg/1, the exception only some zygomycetic pathogens. It equal or even greater than ketoconazole tested....

The therapeutic efficacy of 1% cream and solution lanoconazole, a new imidazole antimycotic agent, in the model cutaneous candidiasis prednisolone-treated guinea pigs was evaluated comparison with that comparable formulations bifonazole. Each preparation topically applied once day for 3 consecutive days, starting on fifth postinfection, quantitative culture study wsa conducted ninth postinfection. Both lanoconazole were much more highly effective terms eradication fungi than bifonazole formulations.

Administration of recombinant human granulocyte-colony stimulating factor (G-CSF), which induces hemopoietic precursor cells to proliferate and differentiate into neutrophils, protected leukopenic mice infected with opportunistic fungi. Mice were injected cyclophosphamide then subcutaneously (s.c.) or intravenously (i.v.) administered 15-120 micrograms G-CSF kg-1 day-1 for 3 days. The number peripheral blood neutrophils in the treated was significantly increased 48 h after administration....

We have previously reported the cases of Candida albicans (C. alb) acid protease (CAAP)-induced atopic asthma. In this study, allergenicity released enzyme CAAP was examined among asthmatic patients with positive immediate skin response to crude C. alb antigen. Among 49 alb, anti-crude IgE antibodies were detected in 40 and anti-CAAP 18. Moreover, anticrude all whom detected. No correlations between IgG both antigens or observed. induced significant T-cell proliferation 20/28 showing Most...

Butenafine is a novel antifungal agent of the class benzylamines. The incidence relapse after topical treatment with butenafine or bifonazole was investigated in guinea pig interdigital tinea pedis model. One percent cream applied on infected site animals for 20 consecutive days starting day 10 postinfection. On 30 posttreatment, infection occurred 11 12 feet treated but only 3 butenafine. lower rate might be attributable to its potent fungicidal activity and long retention time skin.

Two cases of atopic asthma caused by acid protease produced Candida albicans are reported. Both patients had high levels serum IgE antibodies against the and showed positive conjunctival immediate bronchial responses when challenged with protease. Significant histamine release was detected in both their peripheral leukocytes were antigen. These findings clearly that C. is causative allergen asthma.

Amorolfine inhibited the in-vitro growth of Trichophyton mentagrophytes to some extent at a low drug concentration 0.8 ng/ml. Corresponding inhibition, SEM studies revealed slight modification hyphal morphology, i.e. waving surface. These morphological alterations were more extensive with increases in and treatment period: collapsed distorted hyphae exfoliation surface T. occurred 8 ng/ml marked deformation disruption structure 80 amorolfine. TEM thickening cell walls accumulation...

This study aimed to determine whether oral fumonisin exposure contributes the development of psoriasis. Oral administration B1 (FB1, 0.1 mg/kg) or B2 (FB2, was conducted for 10 days, in addition induction psoriatic symptoms through topical application 5% imiquimod cream from day 6 (5 days) female BALB/c mice. The results demonstrated that FB2 significantly exacerbated symptoms, including skin thickness, itching behavior, transepidermal water loss, immune cell infiltration dermis, and...

To investigate the mode of action newly synthesized optically active imidazole compound, NND-502, (-)-(E)-[4-(2, 4-dichlorophenyl)-1,3-dithiolan-2-ylidene]-1-imidazolylacetonit rile, its effect on ergosterol biosynthesis in cell-free extracts Candida albicans was examined and compared with that (S)-enantiomer NND-502 addition to lanoconazole bifonazole, both which are clinically used for treatment dermatomycoses. found interfere by inhibition sterol 14alpha-demethylase, while no interference...