A5036344998- Epigenetics and DNA Methylation
- Prostate Cancer Treatment and Research
- MicroRNA in disease regulation
- Cancer-related gene regulation
- Cancer-related molecular mechanisms research
- Histone Deacetylase Inhibitors Research
- Sirtuins and Resveratrol in Medicine
- RNA modifications and cancer
- Cancer-related Molecular Pathways
- Electrolyte and hormonal disorders
- Coffee research and impacts
- Genomics, phytochemicals, and oxidative stress
- DNA Repair Mechanisms
- Cancer Genomics and Diagnostics
- Biochemical Acid Research Studies
- Bone and Dental Protein Studies
- Chronic Myeloid Leukemia Treatments
- Glioma Diagnosis and Treatment
- Sperm and Testicular Function
- Diet and metabolism studies
- Bladder and Urothelial Cancer Treatments
- Ferroptosis and cancer prognosis
- Prostate Cancer Diagnosis and Treatment
- Potassium and Related Disorders
- PARP inhibition in cancer therapy
Instituto Português de Oncologia Francisco Gentil
2013-2025
IPO Porto
2016-2025
University of Aveiro
2025
Polytechnic Institute of Porto
2013-2017
Universidade do Porto
2012
// Carla Bartosch 1,2,3 , Sara Monteiro-Reis 1,2 Diogo Almeida-Rios Renata Vieira 1 Armando Castro 4 Manuel Moutinho Marta Rodrigues Inês Graça 2 José Lopes 3,4,5,* and Carmen Jerónimo 2,6,* Department of Pathology, Portuguese Oncology Institute-Porto (IPO-Porto), Porto, Portugal Cancer Epigenetics & Biology Group, Research Center, Institute-Porto, 3 Pathology Oncology, Medical Faculty, University Centro Hospitalar São João (CHSJ), 5 IPATIMUP...
// Filipa Quintela Vieira 1, 2 , Pedro Costa-Pinheiro * Diogo Almeida-Rios 5, Inês Graça Sara Monteiro-Reis 5 Susana Simões-Sousa 3, 4 Isa Carneiro Elsa Joana Sousa 1 Maria Godinho 6 Fátima Baltazar Rui Henrique 7, *, # Carmen Jerónimo 7 Cancer Biology and Epigenetics Group – Research Center, Portuguese Oncology Institute Porto, Portugal School of Allied Health Sciences (ESTSP), Polytechnic 3 Life (ICVS), Sciences, University Minho, Braga, ICVS/3B's - PT Government Associate Laboratory,...
Prostate cancer (PCa) is one of the most incident malignancies worldwide. Although efficient therapy available for early-stage PCa, treatment advanced disease mainly ineffective and remains a clinical challenge. microRNA (miRNA) dysregulation associated with PCa development progression. In fact, several studies have reported widespread downregulation miRNAs in which highlights importance studying compounds capable restoring global miRNA expression. The main aim this study was to define...
Prostate cancer (PCa) is a major cause of cancer-related morbidity and mortality worldwide. Although early disease often efficiently managed therapeutically, available options for advanced are mostly ineffective. Aberrant DNA methylation associated with gene-silencing genes common feature PCa. Therefore, inhibitors might constitute an attractive alternative therapy. Herein, we evaluated the anti-cancer properties hydralazine, non-nucleoside methyltransferases (DNMT) inhibitor, in PCa cell...
Background: Current therapeutic strategies for advanced prostate cancer (PCa) are largely ineffective. Because aberrant DNA methylation associated with inappropriate gene-silencing is a common feature of PCa, inhibitors might constitute an alternative therapy. In this study we aimed to evaluate the anti-cancer properties RG108, novel non-nucleoside inhibitor methyltransferases (DNMT), in PCa cell lines. Keywords: Prostate cancer, methyltransferases, proliferation, apoptosis.
Numerous DNA-damaging cellular stresses, including oncogene activation and DNA-damage response (DDR), may lead to senescence. Previous observations linked microRNA deregulation with altered senescent patterns, prompting us investigate whether epigenetic repression of microRNAs expression might disrupt senescence in prostate cancer (PCa) cells.Differential methylation mapping tissues was carried using Infinium HumanMethylation450 BeadChip. After validation analyses a larger series tissues,...
Prostate cancer (PCa) is a major cause of morbidity and mortality. Until now the specific role histone methyltransferases (HMTs) deregulated expression/activity in PCa poorly understood. Herein we aimed to uncover potential oncogenic PRMT6 prostate carcinogenesis. overexpression was confirmed PCa, at transcript protein level. Stable knockdown PC-3 cells attenuated malignant phenotype, increasing apoptosis decreasing cell viability, migration invasion. silencing associated with decreased...
Abstract Upregulation of MYC and miRNAs deregulation are common in prostate cancer (PCa). Overactive may cause miRNAs’ expression through transcriptional post-transcriptional mechanisms epigenetic alterations also involved dysregulation. Herein, we aimed to elucidate the role regulatory network between carcinogenesis. was found upregulated PCa cases matched precursor lesions. MicroRNA’s microarray analysis samples with opposed levels identified significantly overexpressed high- PCa. However,...
Prostate cancer (PCa) is a major cause of morbidity and mortality in men worldwide. MicroRNAs are globally downregulated PCa, especially poorly differentiated tumors. Nonetheless, the underlying mechanisms still elusive. Herein, using combined analysis microRNAs expression genomewide DNA methylation, we aimed to identify epigenetically PCa. We found that miR-152-3p was underexpressed PCa lower levels were associated with promoter hypermethylation accordance TCGA dataset analysis. Functional...
// Tiago Baptista 1,2 , Inês Graça 1,2,4 Elsa J. Sousa Ana I. Oliveira Natália R. Costa Pedro Costa-Pinheiro Francisco Amado 5 Rui Henrique 1,3,6 and Carmen Jerónimo 1,2,6 1 Cancer Epigenetics Group, Research Center, Portuguese Oncology Institute – Porto, Portugal 2 Department of Genetics, 3 Pathology, 4 School Allied Health Sciences ESTSP, Polytechnic Porto Chemistry, Universidade de Aveiro, Campus Universitário Santiago, 6 Pathology Molecular Immunology, Biomedical Abel Salazar, University...
Renal cell carcinoma (RCC) is the 14 th most incident cancer worldwide, and no curative therapeutic options are available for advanced metastatic disease. Hence, new treatment alternatives urgently needed to tackle disease management drug resistance. Herein, we explored use of MLo-1508 as an anti-tumoral agent in RCC further assessed its combination with sunitinib papillary RCC. For that, different lines were treated both drugs, alone or combination, phenotypic assays performed. Moreover,...
Sirtuins are emerging players in cancer biology and other age-related disorders, their putative role bladder (BlCa) remains elusive. Further understanding of disease may allow for generation more effective pathway-based biomarkers targeted therapies. Herein, we aimed to illuminate the sirtuins’ family BlCa evaluate potential as therapeutic targets. SIRT1-7 transcripts protein levels were evaluated a series primary normal mucosa tissues. SIRT7 knockdown was performed through lentiviral...
MGMT downregulation in high-grade gliomas (HGG) has been mostly attributed to aberrant promoter methylation and is associated with increased sensitivity alkylating agent-based chemotherapy. However, HGG harboring 10q deletions also benefit from treatment agents. Because the gene mapped at 10q26, we hypothesized that both epigenetic genetic alterations might affect its expression predict response To test this hypothesis, mRNA levels of were determined by quantitative methylation-specific PCR...
Prostate cancer (PCa), a major cause of cancer-related morbidity and mortality worldwide mostly asymptomatic at earliest stages, is characterized by disruption genetic epigenetic balance. A better understanding how those mechanisms orchestrate disease might improve diagnostic prognostic tools, allowing for improvements in treatment efficacy. Replacement canonical histones, an mechanism, highly conserved among species altered expression histones variants (e.g., MacroH2A1) has been associated...
Deregulated expression of histone deacetylases (HDACs) has been implicated in tumorigenesis. Herein, we investigated class I HDACs bladder urothelial cell carcinoma (BUCC), its prognostic value and biological significance. Significantly increased transcript levels all were found BUCC compared to 20 normal mucosas, these higher lower grade stage tumors. Increased HDAC3 associated with improved patient survival. SiRNA experiments showed decrease viability motility, apoptosis. We concluded that...
Prostate cancer (PCa) is the second leading cause of death among men in Europe and U.S. The metastatic dissemination pattern PCa unique, developing bone metastasis as only site progression, consequently with a prognosis very poor. cells interactions within surrounding environment are critical for tumor growth progression. Secreted protein, acidic rich cysteine (SPARC) described to be involved migration invasion into bone. Three-dimensional (3D) vitro systems that able closely resemble vivo...
Renal Cell Carcinoma (RCC) is on the top 10 of most incident cancers worldwide, being a third patients diagnosed with advanced disease, for which no curative therapies are currently available. Thus, new effective therapeutic strategies urgently needed. Herein, we tested antineoplastic effect newly synthesized 3-nitroflavanones (MLo1302) RCC cell lines. 786-O, Caki2, and ACHN lines were cultured treated 3-nitroflavanones. IC50 values calculated based viability assessed by MTT assay, after 72...