A5100382449- Nanopore and Nanochannel Transport Studies
- Advanced biosensing and bioanalysis techniques
- Biofuel production and bioconversion
- Microfluidic and Capillary Electrophoresis Applications
- Polymer-Based Agricultural Enhancements
- Fuel Cells and Related Materials
- Biosensors and Analytical Detection
- Physics of Superconductivity and Magnetism
- Social Capital and Networks
- Microfluidic and Bio-sensing Technologies
- Medical Imaging and Analysis
- Water Quality Monitoring Technologies
- Advanced Biosensing Techniques and Applications
- Graphene research and applications
- Enzyme Production and Characterization
- Neural Networks and Applications
- Economic Theory and Institutions
- Bone Tissue Engineering Materials
- Molecular Communication and Nanonetworks
- Hemostasis and retained surgical items
- Nanotechnology research and applications
- Financial Distress and Bankruptcy Prediction
- Nanofabrication and Lithography Techniques
- Big Data Technologies and Applications
- Customer Service Quality and Loyalty
Chinese PLA General Hospital
2025
National Clinical Research Center for Digestive Diseases
2025
Ministry of Education of the People's Republic of China
2021-2025
Nanjing University
2008-2024
Peking University
2022
China Pharmaceutical University
2021
Tianjin First Center Hospital
2013-2018
Tianjin Medical University
2013-2018
First Affiliated Hospital of Fujian Medical University
2018
Fujian Medical University
2018
ADVERTISEMENT RETURN TO ISSUEReviewNEXTEnhanced Optical Spectroscopy for Multiplexed DNA and Protein-Sequencing with Plasmonic Nanopores: Challenges ProspectsWang LiWang LiState Key Laboratory of Analytical Chemistry Life Science School Chemical Engineering, Nanjing University, 210023, P. R. ChinaMore by Wang LiView Biography, Juan ZhouJuan ZhouState ZhouView Biographyhttps://orcid.org/0000-0001-9760-5513, Nicolò MaccaferriNicolò MaccaferriDepartment Physics Materials Science, University...
Obtaining sequential and conformational information on proteins is vital to understand their functions. Although the nanopore-based electrical detection can sense single molecule (SM) protein distinguish among different amino acids, this approach still faces difficulties in slowing down translocation improving ionic current signal-to-noise ratio. Here, we observe unfolding multistep of SM cytochrome c (cyt c) through a surface enhanced Raman scattering (SERS) active conical gold nanopore....
Simultaneous detection and structural characterization of protein variants on a single platform are highly desirable but technically challenging. Herein, we present single-molecule spectral system based gold plasmonic nanopore for analyzing two peptides their single-point mutated variants. The enabled the high-throughput acquisition surface-enhanced Raman scattering (SERS) spectra at level by electrically driving analytes into hot spots. Furthermore, statistical method Boolean operations was...
Direct structural and dynamic characterization of protein conformers in solution is highly desirable but currently impractical. Herein, we developed a single molecule gold plasmonic nanopore system for observation allostery, enabling us to monitor translocation dynamics conformation transition proteins by ion current detection SERS spectrum measurement, respectively. Allosteric calmodulin (CaM) was elaborately probed the system. Two CaM were well-resolved at single-molecule level using both...
Enzyme-free single-molecule sequencing has the potential to significantly expand application of nanopore technology DNA, proteins, and saccharides. Despite their advantages over biological nanopores natural suitability for enzyme-free sequencing, conventional solid-state have not yet achieved DNA sequencing. The biggest challenge accuracy using lies in precise control pore size conformity. In this study, we fabricated devices by covering tip a quartz nanopipette with ultrathin...
Structural complexity brings a huge challenge to the analysis of sugar chains. As single-molecule sensor, nanopores have potential provide fingerprint information on saccharides. Traditionally, direct saccharide detection with is hampered by their small size and weak affinity. Here, carbon nitride nanopore device developed discern two types trisaccharide molecules (LeApN SLeCpN) minor structural differences. The resolution LeApN SLeCpN in mixture reaches 0.98, which has never been achieved...
Microfluidic paper-based analytical devices (μPADs), because of their mechanical flexibility, low cost, easy scale-up in manufacturing, small sample volumes and user-friendly properties, show great application prospect bio-medicine, environmental monitor, food industry, drug industry so on. As one kind μPADs, the electrochemical microfluidic (EμPADs) combine advantages μPADs with detection keep attracting more attention. Recently, to improve performance EμPADs, applying nanomaterials for...
A relative stable microenvironment is essential for in vitro cell culture. This paper presents a novel culture device with multiple arrays of perfusion channels (PCs) to maintain the stability and uniformity flow fields. Besides, series regionalized concentration gradients can be generated by adjusting positions inlets. The number circular PCs have been optimized based on simulation results. particle tracking technology has used evaluate changes fields inside chamber as variation velocity...
More anomalous transport behaviors have been observed with the rapid progress in nanofabrication technology and characterization tools. The ions/molecules inside nanochannels can act dramatically different from those bulk systems exhibit novel mechanisms. Here, we reported fabrication of a nanodevice, covalent organic frameworks covered theta pipette (CTP), that combine advantages (TP), framework, field-effect transistors (FETs) for controlling modulating transport. Our results show ammonia,...
Coagulant control plays an important role in water treatment process. Considering the nonlinear and time-delaying property of coagulation, a model coagulant dosage based on RBF neural network is developed. The Gaussian function used for hidden note function, whose centers are adjusted by K-means clustering algorithm. weights output layer obtained RLS. has been trained checked practical data plant. results verify feasibility proposed approach. With optimal algorithm, closed-loop predictive...
Because of nonlinear, time-varying and time-delaying property, itpsilas difficult to model water treatment process by traditional method, so a Takagi-Sugeno fuzzy based on subtractive clustering algorithm is proposed in this paper. Firstly, used partition the input space determine initial values premise parameters rules. Moreover, an improved hybrid study consisting back propagation least square implemented optimize parameters. Finally, method process, simulation results show that it offers...