A5049494407- Bacterial biofilms and quorum sensing
- Bacterial Genetics and Biotechnology
- Vibrio bacteria research studies
- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Bacteriophages and microbial interactions
- Microbial Metabolic Engineering and Bioproduction
- Endoplasmic Reticulum Stress and Disease
- Yersinia bacterium, plague, ectoparasites research
- Lipid Membrane Structure and Behavior
- Plant Pathogens and Fungal Diseases
- Clostridium difficile and Clostridium perfringens research
- Enzyme Production and Characterization
- S100 Proteins and Annexins
- Biochemical and Structural Characterization
- Cystic Fibrosis Research Advances
- Legume Nitrogen Fixing Symbiosis
- Microbial Metabolism and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Antibiotic Resistance in Bacteria
- Legionella and Acanthamoeba research
Université de Rouen Normandie
2019-2025
de Duve Institute
2023-2025
Normandie Université
2019-2025
Communication Bactérienne et Stratégie Anti-infectieuses
2023-2025
ABSTRACT Pseudomonas fluorescens MFE01 is an environmental bacterium characterized by hyperactive type 6 secretion system (T6SS) and a strong emission of volatile organic compounds (VOCs). In previous study, transposition mutant, 3H5, exhibited inactive T6SS altered VOC emission. the interruption trpE gene transposon was insufficient to explain these phenotypes. To determine actual impact this insertion, comparative transcriptomic analysis performed on two-component GacS/GacA, known...
Abstract Predatory bacteria feed upon other in various environments. Bdellovibrio exovorus is an obligate epibiotic predator that attaches on the prey cell surface, where it grows and proliferates. Although mechanisms allowing feeding through envelope are unknown, has been proposed prey’s proteinaceous S-layer may act as a defensive structure against predation. Here, we use time-lapse cryo-electron microscopy to image lifecycle of B. Caulobacter crescentus . We show proliferates by...
Type VI secretion systems (T6SSs) are contractile bacterial multiprotein nanomachines that enable the injection of toxic effectors into prey cells. The Pseudomonas fluorescens MFE01 strain has T6SS antibacterial activity and can immobilise competitive bacteria through T6SS. Hcp1 (hemolysin co-regulated protein 1), a constituent inner tube, is involved in such cell inhibition motility. Paradoxically, disruption hcp1 or tail tssC genes results loss mucoid motile phenotypes MFE01. Here, we...
Biofilms are complex structures formed by a community of microbes adhering to surface and/or each other through the secretion an adhesive and protective matrix. The establishment these requires coordination action between microorganisms powerful communication systems such as quorum-sensing. Therefore, auxiliary bacteria capable interfering with means could be used prevent biofilm formation development. phytopathogen Rhizobium rhizogenes, which causes hairy root disease forms large biofilms...
In many Gram-negative bacteria, virulence, and social behavior are controlled by quorum-sensing (QS) systems based on the synthesis perception of N-acyl homoserine lactones (AHLs). Quorum-quenching (QQ) is currently used to disrupt bacterial communication, as a biocontrol strategy for plant crop protection. this context, Gram-positive bacterium Rhodococcus erythropolis uses catabolic pathway control virulence soft-rot pathogens degrading their AHL signals. This QS signal degradation requires...
The type VI secretion system (T6SS) is a contractile nanomachine widespread in Gram-negative bacteria. T6SS injects effectors into target cells including eukaryotic hosts and competitor microbial thus participates pathogenesis intermicrobial competition. Pseudomonas fluorescens MFE01 possesses single gene cluster that confers biocontrol properties by protecting potato tubers against the phytopathogen Pectobacterium atrosepticum (Pca). Here, we demonstrate functional essential to protect...
The geocaulosphere is home to microbes that establish communication between themselves and others disrupt them. These cell-to-cell systems are based on the synthesis perception of signaling molecules, which best known belong N-acyl-homoserine lactone (AHL) family. Among indigenous bacteria, certain Gram-positive actinobacteria can sense AHLs produced by soft-rot Gram-negative phytopathogens degrade quorum-sensing AHL signals impair expression virulence factors. We mimicked this interaction...
Bacterial communication is a fundamental process used to synchronize gene expression and collective behavior among the bacterial population. The most studied system quorum sensing, cell density system, in which concentration of inductors increases threshold level allowing detection by specific receptors. As result, bacteria can change their coordinated way. While Pseudomonas sensing based on synthesis N -acyl homoserine lactone molecules well studied, volatile organic compounds, although...
Abstract Predatory bacteria feed upon and kill other in various natural environments. Obligate epibiotic predators like Bdellovibrio exovorus consume their prey whilst remaining attached to the outside of prey. How these achieve feeding through cell envelope has not been explored previously. Whereas S-layer is only proposed defensive structure against predatory bacteria, it remains unclear how this thin outer layer might prevent attacks. Similarly, lifecycle B. during predator-prey...