A fully integrated electromechanical resonator is described that based on high mobility piezoelectric semiconductors for actuation and detection of nanoscale motion. We employ the two-dimensional electron gas present at an AlGaN/GaN interface properties this heterostructure to demonstrate a resonant high-electron-mobility transistor enabling strain variation. In device, we take advantage polarization field divergence originated by mechanical flexural modes generating doping. This enables...

Pseudomonas aeruginosa (PA) is a major public health issue due to its impact on nosocomial infections as well cystic fibrosis patient mortality. One of the main concerns ability develop antibiotic resistance. Therefore, inhibition PA virulence has been proposed an alternative strategy tackle based infections. LecA (or PA-IL), galactose binding lectin from PA, involved in virulence. Herein, we aimed at designing high affinity synthetic ligands toward for and understanding key parameters...

The real-life short-term implications of electromagnetic fields (RF-EMF) on cognitive performance and health-related quality life have not been well studied. SPUTNIC study (Study Panel Upcoming Technologies to Non-Ionizing radiation Cognition) aimed investigate possible correlations between mobile phone human health, including cognition, sleep.Adult participants tracked various daily markers RF-EMF exposures (cordless calls, screen time 4 h prior each assessment) as three health outcomes...

Abstract Anti‐infectious strategies against pathogen infections can be achieved through antiadhesive by using multivalent ligands of bacterial virulence factors. LecA and LecB are lectins Pseudomonas aeruginosa implicated in biofilm formation. A series 27 LecA‐targeting glycoclusters have been synthesized. Nine aromatic galactose aglycons were investigated with three different linker arms that connect the central mannopyranoside core. low‐nanomolar ( K d =19 n m , microarray) ligand a...

Antibody microarrays are powerful and high-throughput tools for screening identifying tumor markers from small sample volumes of only a few microliters. Optimization surface chemistry spotting conditions crucial parameters to enhance antibodies' immobilization efficiency maintain their biological activity. Here, we report the implementation an antibody microarray detection involved in colorectal cancer. Three-dimensional microstructured glass slides were functionalized with three different...

A library of 24 new mannose-centered tetragalactoclusters with four different linkers (di- and triethyleneglycol phosphodiester or phosphorothioate linkages) six aromatic aglycons (O-phenyl, S-phenyl, O-benzyl, S-benzyl, O-biphenyl O-naphthyl) was synthesized. Their interactions LecA were evaluated on a DNA Directed Immobilization (DDI) based glycocluster array allowing the determination their IC50 against lactose evaluation dissociation constant (Kd). Finally, docking simulations confirm...

Abstract Lectin A (LecA) from Pseudomonas aeruginosa is an established virulence factor. Glycoclusters that target LecA and are able to compete with human glycoconjugates present on epithelial cells promising candidates treat P. infection. family of 32 glycodendrimers generation 0 1 based a bifurcated bis‐galactoside motif have been designed interact LecA. The influences both the central multivalent core aglycon these their affinity toward evaluated by use microarray technique, qualitatively...

Surface chemical functionalization is used in analytical tools to immobilize biomolecules that will capture a specific analyte and also reduce the nonspecific adsorption. Silane monolayers are widely functionalize silica surfaces. Their interfacial properties linked silane organization. Here, we study, by molecular dynamics simulations, effects of molecule head-group charge, alkyl chain length, surface coverage on structure monolayers. Four molecules investigated:...

Pseudomonas aeruginosa (PA) is an opportunistic bacterium involved in 10-30% of nosocomial diseases. It causes severe lung injury to cystic fibrosis patients, often leading patient death. PA strains are multidrug resistant, thus making the design new therapeutics a challenge for public health. One promising therapeutic option glycoclusters that target virulence factor PA. LecA galactose-specific lectin might be adhesion and biofilm formation by The DNA-directed immobilization (DDI)...

Abstract Due to the ability of Pseudomonas aeruginosa (PA) develop antibiotic resistances, alternative therapeutic strategies have been proposed. Among others, carbohydrate multivalent molecules targeting lectin‐based virulent factors widely reported in particular those LecA. LecA is a tetravalent galactose specific lectin involved biofilm formation and cell internalization. Herein, we report synthesis 36 galactoclusters built from galactosides with aromatic non‐aromatic aglycons an...

The Gram negative bacterium Pseudomonas aeruginosa (PA) is an opportunistic that causes severe and chronic infection of immune-depressed patients. It has the ability to form a biofilm gives selective advantage bacteria with respect antibiotherapy host defenses. Herein, we have focused on tetrameric soluble lectin which involved in adherence cells, formation, cytotoxicity. binds l-fucose, d-mannose glycan exposing terminal fucose or mannose. Using competitive assay microarray, 156...

Titanium tungsten (TiW) films (200 nm thick) were cleaned by oxygen plasma, and the resulting oxidized surfaces functionalized 3-aminopropylphosphonic acid (APPA), 3-ethoxydimethylsilylpropylamine (APDMES), or dopamine (DA) to form three different organolayers. The organolayers characterized X-ray photoelectron spectroscopy, time-of-flight secondary ion mass spectrometry, Fourier transform infrared spectroscopy analyses. stability of each organolayer was investigated. Our results suggested...

Desorption ionization on silicon mass spectrometry (DIOS-MS) enables high throughput analysis of low-molecular-weight biomolecules. However, detection metabolite biomarkers in complex fluids such as plasma requires sample pretreatment, limiting clinical application. Here, we show that porous silicon, chemically modified using monolayers n-propyldimethylmethoxysilane molecules, is a good candidate for fingerprinting lysophosphatidylcholine (lysoPC) plasma, without DIOS-MS-based diagnosis...

Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-ToF MS) is a promising strategy for clinical diagnosis based on metabolite detection. However, several bottlenecks (such as the lack of reproducibility in analysis, presence an important background low-mass range, and organic matrix some molecules) prevent its transfer to cases. These limitations can be addressed by using nanoporous silicon surfaces chemically functionalized with silane monolayers. In present...

The evolution of nanobiosensors stresses the need for multi-material nanopatterned surfaces to enhance sensing performances. Titanium tungsten (TiW) has been mastered and routinely implemented in nanoelectronic devices, a reproducible way at industrial production scales. Such material may be envisioned use (bio)chemical sensors, but surface functionalization such yet studied. In present article, orthogonal chemical patterned Au on TiW substrates explored first time. Surface...

Oligo ethylene glycol layers are widely used in biosensor applications, mainly for their anti-fouling abilities. Such surfaces often characterized by X-ray photoelectron spectroscopy (XPS) as this method allows a precise determination of the surface chemical composition. We show herein that X-rays during XPS characterization quickly and significantly degrade oligo immobilized onto silica substrates. It is therefore necessary to introduce correcting factor assess true (i.e. without...

In this paper, we present experimental results on AlSb/InAs HEMTs with deep and shallow mesa using wet dry etching techniques respectively. Similar electrical have been obtained both techniques.

We report here a new microfluidic method allowing for the quantification of human epidermal growth factor receptor 2 (HER2) expression levels from formalin-fixed breast cancer tissues. After partial extraction proteins tissue slide, extract is routed to an antibody (Ab) microarray HER2 titration by fluorescence. Then HER2-expressing cell area evaluated immunofluorescence (IF) staining slide and used normalize fluorescent signal measured Ab microarray. The number gene copies fluorescence in...