A5005565609- Osteoarthritis Treatment and Mechanisms
- Knee injuries and reconstruction techniques
- Helicobacter pylori-related gastroenterology studies
- Metabolomics and Mass Spectrometry Studies
- Adipose Tissue and Metabolism
- Pancreatic function and diabetes
- Lower Extremity Biomechanics and Pathologies
- 3D Printing in Biomedical Research
- Diabetes and associated disorders
- Galectins and Cancer Biology
- Veterinary medicine and infectious diseases
- Exercise and Physiological Responses
- Muscle metabolism and nutrition
- High Altitude and Hypoxia
- Spaceflight effects on biology
- Phagocytosis and Immune Regulation
- Congenital heart defects research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Proteoglycans and glycosaminoglycans research
- Silk-based biomaterials and applications
- Diabetic Foot Ulcer Assessment and Management
- Clostridium difficile and Clostridium perfringens research
- Total Knee Arthroplasty Outcomes
- Antimicrobial Peptides and Activities
- Microbial Metabolites in Food Biotechnology
Montana State University
2020-2025
Vanderbilt University Medical Center
2014
Vanderbilt University
2008-2012
University of Georgia
2004-2008
Osteoarthritis (OA) is a chronic joint disease with heterogenous metabolic pathology. To gain insight into OA-related metabolism, metabolite extracts from healthy (n = 11) and end-stage osteoarthritic cartilage 35) were analyzed using liquid chromatography–mass spectrometry metabolomic profiling. Specific metabolites pathways, including lipid amino acid differentially regulated in osteoarthritis-derived cartilage. The detected alterations acids lipids highlighted key differences bioenergetic...
Helicobacter pylori FlgR activates transcription with sigma54-RNA polymerase holoenzyme (sigma54-holoenzyme) from at least five flagellar operons. Activators of sigma54-holoenzyme generally bind enhancer sequences located >70 bp upstream the promoter and contact bound through DNA looping to activate transcription. H. lacks carboxy-terminal DNA-binding domain present in most sigma54-dependent activators. As little as 42 flaB 26 sequence downstream transcriptional start site were sufficient...
Articular cartilage is comprised of two main components, the extracellular matrix (ECM) and pericellular (PCM). The PCM helps to protect chondrocytes in from mechanical loads, but patients with osteoarthritis, weakened, resulting increased chondrocyte stress. As are responsible for synthesis maintenance, it important understand how loads affect cellular responses chondrocytes. Many studies have examined
Osteoarthritis occurs frequently after joint injury. Currently, osteoarthritis is diagnosed by radiographic changes that are typically found the disease has progressed to multiple tissues. The primary objective was compare potential metabolomic biomarkers of injury between synovial fluid and serum in a mouse model posttraumatic osteoarthritis. secondary gain insight into pathophysiology examining profiles Twelve-week-old adult female C57BL/6 mice (n = 12) were randomly assigned control, Day...
We hypothesized that daily exercise promotes joint health by upregulating anti-inflammatory mediators via adaptive molecular and metabolic changes in the infrapatellar fat pad (IFP). tested this hypothesis conducting time-resolved analyses between 1 14 days of voluntary wheel running C57BL/6J mice. IFP structure cellularity were evaluated histomorphology, picrosirius red collagen staining, flow cytometry analysis stromal vascular fraction cells. Joint inflammation metabolism multiplex gene...
Abstract Human chondrocytes are responsible for cartilage repair and homeostasis through metabolic production of precursors to collagen other matrix components. This metabolism is sensitive both the availability media energy sources as well local temperature. Central carbon metabolites such glucose glutamine essential not only producing energetic compounds ATP NADH, but also assembling aggrecan from non-essential amino acid precursors. The rate at which this takes place directly relates...
Helicobacter pylori colonizes the human stomach and is etiological agent of peptic ulcer disease. All three H. strains that have been sequenced to date contain a potential operon whose products share homology with subunits acetone carboxylase (encoded by acxABC) from Xanthobacter autotrophicus strain Py2 Rhodobacter capsulatus B10. Acetone catalyzes conversion acetoacetate. Genes upstream putative acxABC encode enzymes convert acetoacetate acetoacetyl-CoA, which metabolized further generate...
ABSTRACT Objective Osteoarthritis (OA) is a chronic joint disease with heterogenous metabolic pathology. To gain insight into OA-related metabolism, healthy and end-stage osteoarthritic cartilage were compared metabolically to uncover disease-associated profiles, classify OA-specific endotypes, identify targets for intervention the diverse populations of individuals affected by OA. Design Femoral head (n=35) from osteoarthritis patients collected post-total arthroplasty. Healthy (n=11) was...
Abstract Articular chondrocytes synthesize and maintain the avascular aneural articular cartilage. In vivo these cells are surrounded by a 3D pericellular matrix (PCM) containing predominantly collagen VI. The PCM protects facilitates mechanotransduction, stiffness is critical in transmitting biomechanical signals to chondrocytes. Various culture systems with different hydrogels have been used encapsulate for culture, but many lack either or of cartilage matrix. Here, we demonstrate that...
Abstract Posttraumatic osteoarthritis (PTOA) commonly develops following anterior cruciate ligament (ACL) injuries, affecting around 50% of individuals within 10–20 years. Recent studies have highlighted early changes in subchondral bone structure after ACL injury adolescent or young adult mice, which could contribute to the development PTOA. However, injuries do not only occur life. Middle‐aged and older patients also experience PTOA, but whether aged responds rapidly is unknown. This study...
Abstract The mechanism by which chondrocytes respond to reduced mechanical loading environments and the subsequent risk of developing osteoarthritis remains unclear. This is particular concern for astronauts. In space joint forces during prolonged microgravity (10 -6 g ) exposure could lead (OA), compromising quality life post-spaceflight. this study, we encapsulated human in an agarose gel similar stiffness pericellular matrix mimic cartilage microenvironment. We then exposed...
Abstract One of the main components articular cartilage is chondrocyte's pericellular matrix (PCM), which critical for regulating mechanotransduction, biochemical cues, and healthy development. Here, individual primary human chondrocytes (PHC) are encapsulated cultured in 50 µm diameter alginate microgels using drop‐based microfluidics. This unique culturing method enables PCM formation manipulation cells. Over ten days, observed autofluorescence imaging, elastic moduli isolated cells...
Several Helicobacter pylori flagellar genes require σ54 for their transcription. Predicted H. pyloriσ54-dependent promoters display a preference A at position −23 instead of C or T as occurs in from most other bacteria. Substitution the flaB promoter with did not effect expression flaB′–′xylE reporter gene pylori, whereas G substitutions this drastically reduced expression. Results gel mobility shift assays that used DNA probes corresponding to core sequences and pyloriσ54 protein fused...
Abstract The mechanism by which chondrocytes respond to reduced mechanical loading environments and the subsequent risk of developing osteoarthritis remains unclear. This is particular concern for astronauts. In space joint forces during prolonged microgravity (10 −6 g) exposure could lead (OA), compromising quality life post-spaceflight. this study, we encapsulated human in an agarose gel similar stiffness pericellular matrix mimic cartilage microenvironment. We then exposed...
Abstract Joint injury is a risk factor for post-traumatic osteoarthritis. However, metabolic and microarchitectural changes within the joint post-injury in both sexes remain unexplored. This study identified tissue-specific spatially-dependent signatures male female mice using matrix-assisted laser desorption ionization-mass spectrometry imaging (MALDI-MSI) LC-MS metabolomics. Male C57Bl/6J were subjected to non-invasive injury. Eight days post-injury, serum, synovial fluid, whole joints...
Abstract Osteoarthritis, the most common degenerative joint disease, occurs more frequently in joints that have sustained injury. Currently, osteoarthritis is diagnosed with imaging finds radiographic changes after disease has already progressed to multiple tissues. The primary objective of this study was compare potential metabolomic biomarkers injury between synovial fluid and serum a mouse model post-traumatic osteoarthritis. secondary gain insight into pathophysiology by examining...
Abstract Human articular cartilage is comprised of two main components, the extracellular matrix (ECM) and pericellular (PCM). The PCM helps to protect chondrocytes in from mechanical loads, but patients with osteoarthritis, weakened resulting increased chondrocyte stress. As are responsible for synthesis maintenance, it important understand how loads affect cellular responses chondrocytes. Many studies have examined response vitro loading by embedding stiff agarose. However, these...