A5043576620- Microbial Metabolic Engineering and Bioproduction
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Amino Acid Enzymes and Metabolism
- Biofuel production and bioconversion
- RNA and protein synthesis mechanisms
- Fungal and yeast genetics research
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Biochemical and Molecular Research
Universidad Autónoma Metropolitana
2020-2023
Universidad Nacional Autónoma de México
2009-2013
Anthranilate is an aromatic amine used industrially as intermediate for the synthesis of dyes, perfumes, pharmaceuticals and other classes products. Chemical anthranilate unsustainable process since it implies use nonrenewable benzene generation toxic by-products. In Escherichia coli synthesized from chorismate by synthase (TrpED) then converted to phosphoribosyl transferase continue tryptophan biosynthetic pathway. With purpose generating a microbial strain production glucose, E. W3110...
Phosphoenolpyruvate (PEP) is a precursor involved in the biosynthesis of aromatics and other valuable compounds Escherichia coli. The PEP:carbohydrate phosphotransferase system (PTS) major glucose transport largest PEP consumer. To increase intracellular availability for production purposes, mutant strains E. coli JM101 devoid ptsHIcrr operon (PB11 strain) have been previously generated. In this derivative, growth rate on decreased significantly. A laboratory evolved strain derived from PB11...
Impact statement Plasmid DNA manufacture is an essential step to produce gene therapy agents and next‐generation vaccines. However, little attention has been paid toward developing alternative replicons that can be coupled with large‐scale production conditions. Our results demonstrate the miniR1 replicon efficiently induced by oxygen limitation when a copy of regulatory protein RepA under control microaerobic promoter used. The are potentially attractive for industrial applications.