A5039762089- Marine Invertebrate Physiology and Ecology
- Ion channel regulation and function
- Neurobiology and Insect Physiology Research
- Marine Toxins and Detection Methods
- Nicotinic Acetylcholine Receptors Study
- Marine Sponges and Natural Products
- Protein Hydrolysis and Bioactive Peptides
- Venomous Animal Envenomation and Studies
- Antimicrobial Peptides and Activities
- Biochemical effects in animals
- Photoreceptor and optogenetics research
- Biochemical Analysis and Sensing Techniques
- Ion Transport and Channel Regulation
- Echinoderm biology and ecology
- Aquaculture disease management and microbiota
- Chemical Synthesis and Analysis
- Sulfur-Based Synthesis Techniques
Russian Academy of Sciences
2025
Pacific Institute of Bioorganic Chemistry. GB Elyakova Far Eastern Branch of the Russian Academy of Sciences
2018-2023
Far Eastern Branch of the Russian Academy of Sciences
2018
Parkinson's disease (PD) is a socially significant disease, during the development of which oxidative stress and inflammation play role. Here, we studied neuroprotective effects four Kunitz-type peptides from Heteractis crispa magnifica sea anemones against PD inductors. The peptide HCIQ1c9, was obtained for first time, inhibited trypsin less than other due to unfavorable interactions Arg17 with Lys43 in enzyme. Its activity reduced by up 70% over temperature range 60-100 °C, while HCIQ2c1,...
Toxins modulating NaV channels are the most abundant and studied peptide components of sea anemone venom. Three type-II toxins, δ-SHTX-Hcr1f (= RpII), RTX-III, RTX-VI, were isolated from Heteractis crispa. RTX-VI has been found to be an unusual analog RTX-III. The electrophysiological effects toxins on nine subtypes investigated for first time. mainly affect inactivation mammalian expressed in central nervous system (NaV1.1–NaV1.3, NaV1.6) as well insect arachnid (BgNaV1, VdNaV1). absence...
Acid-sensing ion channels (ASICs) have been known as sensors of a local pH change within both physiological and pathological conditions. ASIC-targeting peptide toxins could be potent molecular tools for ASIC-manipulating in vitro, pathology treatment animal test studies. Two sea anemone toxins, native Hmg 1b-2 recombinant 1b-4, related to APETx-like peptides, inhibited the transient current component human ASIC3-Δ20 expressed Xenopus laevis oocytes, but only rat ASIC3 current. The 1b-4...
Currently, five peptide modulators of acid-sensing ion channels (ASICs) attributed to structural class 1b sea anemone toxins have been described. The APETx2 toxin is the first and most potent ASIC3 inhibitor, so its homologs from anemones are known as APETx-like peptides. We discovered that two peptides Heteractis crispa, Hcr 1b-3 1b-4, demonstrate different effects on rASIC1a rASIC3 currents. While inhibits both investigated ASIC subtypes with IC50 4.95 ± 0.19 μM for 17 5.8 rASIC3, 1b-4 has...
Sea anemones are a rich source of biologically active compounds. Among approximately 1100 species described so far, Heteractis crispa species, also known as sebae anemone, is native to the Indo-Pacific area. As part its venom components, Hcr 1b-2 peptide was first an ASIC1a and ASIC3 inhibitor. Using Xenopus laevis oocytes two-electrode voltage-clamp technique, in present work we describe remarkable lack selectivity this toxin. Besides acid-sensing ion channels previously described,...
The nicotinic acetylcholine receptors (nAChRs) are prototypical ligand-gated ion channels, provide cholinergic signaling, and modulated by various venom toxins drugs in addition to neurotransmitters. Here, four APETx-like toxins, including two new named Hmg 1b-2 Metox 1b-5, were isolated from the sea anemone Heteractis magnifica characterized as novel nAChR ligands acid-sensing channel (ASIC) modulators. All peptides competed with radiolabeled α-bungarotoxin for binding Torpedo californica...
Diabetes mellitus is one of the most serious diseases our century. The drugs used are limited or have side effects. search for new sources compounds effective treatment relevant. Magnificamide, a peptide inhibitor mammalian α-amylases, isolated from venom sea anemone Heteractis magnifica, can be control postprandial hyperglycemia in diabetes mellitus. Using RACE approach, seven isoforms magnificamide were detected H. magnifica tentacles. exon–intron structure genes was first established, and...
A novel peptide AnmTX Sco 9a-1 with the β-hairpin fold was isolated from swimming sea anemone Stomphia coccinea (Actinostolidae family). The consists of 28 amino acid residues, including modified hydroxyproline residue, and its measured molecular mass is 2960 Da. not toxic on mice; however, it stimulated their exploratory motivation active search behavior, demonstrated an anti-anxiety effect. at doses 0.1 1 mg/kg reduced volume edema during 24 h better than nonsteroidal anti-inflammatory...