A5014859305- Immune Cell Function and Interaction
- CRISPR and Genetic Engineering
- CAR-T cell therapy research
- Silicon Carbide Semiconductor Technologies
- Cancer Immunotherapy and Biomarkers
- Virus-based gene therapy research
- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
University Children's Hospital Tübingen
2024
Relapse and graft-versus-host disease (GVHD) are the main causes of death after allogeneic hematopoietic cell transplantation (HCT). Preclinical murine models clinical data suggest that invariant natural killer T (iNKT) cells prevent acute chronic GVHD. In addition, iNKT crucial for efficient immune responses against malignancies contribute to reduced relapse rates transplantation. Chimeric antigen receptors (CAR) redirect effector surface antigens enhance killing target cells. With this...
Non-viral gene editing via CRISPR-Cas12a offers an alternative to Cas9-based methods, providing better targeting of AT-rich regions, simplified guide RNA manufacturing, and high specificity. However, the efficacy outcomes is subject various factors, with tem-plate format playing a crucial role. Currently, predominant non-viral template for inducing homology-directed repair (HDR) after nuclease-induced DNA breaks double-stranded (dsDNA), which toxic when transfected at doses. Previous studies...