- Glycosylation and Glycoproteins Research
- Salivary Gland Disorders and Functions
- Geological and Geochemical Analysis
- Carbohydrate Chemistry and Synthesis
- Infant Nutrition and Health
- Geology and Paleoclimatology Research
- Streptococcal Infections and Treatments
- Probiotics and Fermented Foods
- Escherichia coli research studies
- Galectins and Cancer Biology
- Geological and Geophysical Studies
- Meteorological Phenomena and Simulations
- Toxin Mechanisms and Immunotoxins
- Digestive system and related health
- Seed Germination and Physiology
- Agricultural Systems and Practices
- Weed Control and Herbicide Applications
- earthquake and tectonic studies
- Geomagnetism and Paleomagnetism Studies
- Microbial Metabolites in Food Biotechnology
- Silkworms and Sericulture Research
- Oral microbiology and periodontitis research
- Enzyme Production and Characterization
- Agricultural Science and Fertilization
- Earthquake and Disaster Impact Studies
Ibaraki University
2015-2024
National Agriculture and Food Research Organization
2022
Kyushu Okinawa Agricultural Research Center
2018-2022
Miyazaki Agricultural Research Institute
2021
Western Region Agricultural Research Center
2004-2018
Osaka University
1977-2009
Shikoku (Japan)
2008-2009
Institute of Livestock and Grassland Science
1999-2002
Toyobo (Japan)
1991-1995
National Institute for Agro-Environmental Sciences
1993
This paper presents data on reactions of murine macrophages with a variety lectins, special focus Griffonia simplicifolia I-B4 isolectin, the only lectin we tried that distinguishes stimulated from resident populations. Specificity I reaction carbohydrate determinants at cell surface is shown by (i) ability alpha-galactosidase treatment intact cells to abolish all binding whereas beta-galactosidase has no effect binding, (ii) methyl alpha-D-galactopyranoside completely inhibit having (iii)...
A high level protein synthesis is one of the characteristics cancer cells. The aim this study to show contribution eukaryotic elongation factor 2 (eEF2), which plays an essential role in polypeptide chain step, tumorigenesis gastrointestinal cancers. In present study, we demonstrated by using immunohistochemistry that eEF2 was overexpressed 92.9% (13 14) gastric and 91.7% (22 24) colorectal No mutations were found any exons gene six Knockdown eEF2-specific short-hairpin RNA (shEF2) inhibited...
Abstract The 220–250 km 3 (VEI 7) caldera-forming eruption of Maninjau (West Sumatra, Indonesia) in 52 ±3 ka played a major role for changing the landscape Padang Volcanic Field. Besides forming thick and widespread ignimbrite plateau (especially vicinity Bukttinggi), successfully exposed stratigraphy pre-caldera rocks which predominantly constructed lava (hereafter referred to as somma lava). Since our knowledge regarding relationship between lavas silicic activity is lacking, we aimed...
Abstract The 74 ka Youngest Toba Tuff (YTT) super-eruption is one of the largest caldera-forming eruptions on Earth. Thick welded facies (~100 m) YTT are exposed 30 km northwest source caldera. In this outcrop, an entablature columnar joints, consisting multiple domains can be well observed. domain cracked by a vertical master joint (cooling plane), from which chilled margin and many joints developed laterally. study, paleomagnetic method was applied to evaluate cooling history genetic...
A fourth lectin (GS IV), a serologically Lewis b (Leb)active binding lectin, was isolated from Griffonia simplicifolia seeds by affinity chromatography on an Leb (a-L-fucose-(1 + 2)-P-~-Gal-(1-+ 3)-[a-~-fucose-(l+ 4)] -P-D-GlcNAc)-active fragment coupled to Synsorb, followed elution with 0.1 N acetic acid.The purified shown be homogeneous polyacrylamide gel electrophoresis and analytical column Sephadex G-200.A glycoprotein containing mannose, N-acetylglucosamine, fucose, xylose (4:2:1:1),...
It is postulated that an initial step in dental plaque formation the adherence of oral bacteria to salivary pellicle. Recently, we have found a proline-rich and basic glycoprotein (MGP) from human parotid saliva, which successfully purified by Concanavalin A-Sepharose affinity chromatography, binds some streptococci such as S. mitis sanguis. This paper deals with inhibitors affect binding MGP sanguis ATCC 10557. The assay for ability radioactive bacterial cells was performed incubation...
"Phreatoplinian" is an explosive phreatomagmatic eruption style that defined by the fragmentation of magma and widespread dispersal resulting fine ash accretionary lapilli. These eruptions pose significant future risks at caldera volcanoes host lakes abundant groundwater. There have been no direct observations a phreatoplinian eruption, therefore, constraining detailed mechanisms sequences such events relies on studying deposits previous eruptions. In order to advance our understanding these...
P revious studies have indicated that a galactosephilic component present on the bacterial cell surfaces of Streptococcus sanguis ATCC 10557 may be responsible for salivary glycoprotein‐medtated binding cells. The purpose this study was to investigate purification and characterization surface from S. 10557. A involving fibrils isolated by techniques freezing thawing, purified an affinity chromatography β‐ d ‐galactose binding‐Bio‐Gel P‐2 followed gel filtrations Bio‐Gel P‐150 P‐30. Both disk...
Abstract We have carried out paleomagnetic analyses of tephras from the Taupō eruption, one most violent eruptions on Earth in past 5000 years. Pyroclastic deposits were collected with 7 cm 3 cubes pushed into each unit eruption sequence, consisting airfall units and overlying ignimbrite. Where possible, we targeted fine-ash layers matrix, as lapilli sized material can significantly affect quality analysis. The samples oriented using a collection device specially designed to maximize...
The conformational study of three proline-rich polypeptides human whole saliva, with known primary structures, was performed by CD and 1H-n.m.r. spectra measurements. All these contained more than four consecutive prolyl residues in their amino acid sequences. occurrence the poly-L-proline form II conformation structures demonstrated two polypeptides. continuous third suggested to take same structure as others.
A study was conducted to determine the length of exposure heat necessary kill water-imbibing weed seeds at 55 and 60°C. The examination covered ten upland species: Solanum carolinense L., S. americanum Mill., Abutilon theophrasti Medic., Phytolacca americana Amaranthus spinosus A. patulus Bertoloni, Persicaria lapathifolia (L.) F. Gray, Panicum dichotomiflorum Michx, Echinochloa crus-galli Beauv. var. Digitaria ciliaris (Retz.) Koeler. All each species were killed by 55°C for 72hr 60°C 24hr...
alpha-L-Fucosidase acting on naturally occurring substrates was highly purified from the growth culture of Streptococcus sanguis ATCC 10557. The molecular weight enzyme approximately 120,000 and optimal pH at 5.5. showed specificity toward linkage alpha-(1 leads to 2) fucosides in oligosaccharides glycoproteins. released L-fucose glycoprotein human parotid saliva.
Abstract To constrain the age and duration of large-scale caldera-forming eruptions, we measured paleomagnetic directions pyroclastic deposits from 30 ka Aira caldera eruption sequence, 60–45 Iwato flow around in southern Kyushu, Japan. The sequence consists Osumi pumice fall (OS), Tarumizu deposit (TM), Ito ignimbrite (IT) co-ignimbrite ash (AT), ascending order. Oriented samples were collected by drilling for OS, TM welded ignimbrites, cubing non-welded (unconsolidated) deposit. We...
An alkaline phosphatase-conjugated 29-base oligonucleotide probe was developed to detect the gene encoding bundle-forming pilus of enteropathogenic Escherichia coli. The sensitivity and specificity versus results localized adherence in HEp-2 cell assay fluorescent actin staining positivity were 95.7 100%, respectively.
The temperature range of germination, thermal time and the effects fluctuation on horsenettle (Solanum carolinense L.) seeds were investigated. Base (Tb) (θ) to germination for treated with gibberellin calculated by Garcia-Huidobro's method. Tb was estimated be between 14-16°C. Maximum surmised 45-50°C from a graph. Gompertz function adequately described relationship θ fraction (%), but modification equation needed in some cases. percentage exposed diurnal an upper limit 15-30°C amplitudes...
熊本地震により熊本市東区秋津地区にある54...
This study developed a farmland meteorology estimation system to support precise cultivation management. The is suitable for application even in areas of hilly, mountainous, or sloping terrain. provides daily maximum, minimum, and mean air temperatures, relative humidity, accumulations solar radiation, reference evapotranspiration, precipitation, together with 6-hourly predictions precipitation. These data are estimated from the Automated Meteorological Data Acquisition System (AMeDAS)...
Galactosyltransferase is human parotid saliva catalyzed the transfer of galactose from uridine 5'-diphospho (UDP)-galactose to D-glucose in presence exogenous alpha-lactalbumin. Some albumins other than alpha-lactalbumin did not have an effect on appearance lactosesynthesizing activity.