A5025736201- Helicobacter pylori-related gastroenterology studies
- Cardiac electrophysiology and arrhythmias
- Ion channel regulation and function
- Galectins and Cancer Biology
- Cardiomyopathy and Myosin Studies
- Microbial Metabolites in Food Biotechnology
- Genomics and Chromatin Dynamics
- Immune Response and Inflammation
- Inflammasome and immune disorders
- Gastric Cancer Management and Outcomes
- RNA Research and Splicing
- Veterinary medicine and infectious diseases
- Gastroesophageal reflux and treatments
- Clostridium difficile and Clostridium perfringens research
- Escherichia coli research studies
- Dermatology and Skin Diseases
- Enterobacteriaceae and Cronobacter Research
- Genomic variations and chromosomal abnormalities
Vanderbilt University Medical Center
2023-2025
Vanderbilt University
2019-2021
University of Tennessee at Knoxville
2015
Bacterial type IV secretion systems (T4SSs) have been phylogenetically grouped into two subfamilies. The T4ASSs, represented by the Agrobacterium tumefaciens VirB/VirD4 T4SS , include “minimized” machines assembled from 12 VirB- and VirD4-like subunits compositionally larger such as Helicobacter pylori Cag . T4BSSs encompass closely related in subunit composition to Legionella pneumophila Dot/Icm Here, we present structures of native mutant H. determined situ cryoelectron tomography. We...
KCNE1 encodes a 129-residue cardiac potassium channel (IKs) subunit. variants are associated with long QT syndrome and atrial fibrillation. However, most have insufficient evidence of clinical consequences thus limited utility. In this study, we leveraged the power variant effect mapping, which couples saturation mutagenesis high-throughput sequencing, to ascertain function thousands protein-coding variants. We comprehensively assayed cell surface expression (2554/2709 possible...
Abstract Herpes simplex 1 infection of the eye can cause blindness with lesions in corneal stroma largely attributable to inflammatory events that include components both adaptive and innate immunity. Several immune responses are triggered by herpes 1, but it is unclear how such relate subsequent development stromal keratitis. In this study, we compared outcome ocular mice unable express NLRP3 because gene knockout (NLRP3−/−) wild-type mice. The NLRP3−/− developed more-severe earlier...
Background: KCNQ1 loss of function variants are thought to cause type 1 long QT syndrome by reducing IKs. However, we have recently reported that pharmacologic block IKs in human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) produced minimal increases action potential duration at 90% repolarization (APD90), while genetic markedly prolonged APD90. We sought here define mechanisms underlying APD prolongation KCNQ1. Methods: studied iPSC-CMs from population controls, an...
Helicobacter pylori colonizes the stomach in about half of world's population. H. strains containing cag pathogenicity island (cag PAI) are associated with a higher risk gastric adenocarcinoma or peptic ulcer disease than PAI-negative strains. The PAI encodes type IV secretion system (T4SS) that mediates delivery CagA effector protein as well nonprotein bacterial constituents into epithelial cells. pylori-induced nuclear factor kappa-light-chain-enhancer activated B cells (NF-κB) activation...
The Helicobacter pylori Cag type IV secretion system (T4SS) translocates the effector protein CagA and nonprotein bacterial constituents into host cells. In this study, we infected Mongolian gerbils with an H. strain in which expression of cagUT operon (required for T4SS activity) is controlled by a TetR/tetO system. Transcript levels cagU were significantly higher gastric tissue from pylori-infected animals receiving doxycycline-containing chow (to derepress than control drug-free chow. At...
Both Helicobacter pylori infection and a high-salt diet are risk factors for gastric cancer. We previously showed that mutation in fur (encoding the ferric uptake regulator variant Fur-R88H) was positively selected H. strains isolated from experimentally infected Mongolian gerbils receiving diet.
Abstract Background KCNE1 encodes a 129-residue cardiac potassium channel (I Ks ) subunit. variants are associated with long QT syndrome and atrial fibrillation. However, most have insufficient evidence of clinical consequences thus limited utility. Results Here, we demonstrate the power variant effect mapping, which couples saturation mutagenesis high-throughput sequencing, to ascertain function thousands protein coding variants. We comprehensively assayed cell surface expression...
Helicobacter pylori genomes encode over 60 predicted outer membrane proteins (OMPs). Several OMPs in the Hop family act as adhesins, but functions of most are unknown. To identify