A5035434314- Influenza Virus Research Studies
- RNA and protein synthesis mechanisms
- interferon and immune responses
- Viral gastroenteritis research and epidemiology
- Bacteriophages and microbial interactions
- Animal Disease Management and Epidemiology
- Herpesvirus Infections and Treatments
- Virology and Viral Diseases
- Yersinia bacterium, plague, ectoparasites research
- Monoclonal and Polyclonal Antibodies Research
- Viral Infections and Vectors
- Poxvirus research and outbreaks
- Virus-based gene therapy research
- Viral Infections and Outbreaks Research
- Cancer Mechanisms and Therapy
- Respiratory viral infections research
- Chromatin Remodeling and Cancer
University Medical Center Freiburg
2014-2024
University of Freiburg
2016-2024
Lysine acetylation is a post-translational modification known to regulate protein functions. Here we identify several sites of the influenza A virus nucleoprotein (NP), including lysine residues K77, K113 and K229. Viral growth mutant encoding K229R, mimicking non-acetylated NP residue, severely impaired compared wildtype or viruses K77R K113R. This attenuation not result decreased polymerase activity, altered expression disordered vRNP co-segregation but rather caused by particle release....
Two novel influenza A-like viral genome sequences have recently been identified in Central and South American fruit bats provisionally designated "HL17NL10" "HL18NL11." All efforts to isolate infectious virus from or generate these viruses by reverse genetics failed date. Recombinant vesicular stomatitis (VSV) encoding the hemagglutinin-like envelope glycoproteins HL17 HL18 place of VSV glycoprotein were generated identify cell lines that are susceptible bat entry. More than 30 derived...
Abstract Packaging of the eight genomic RNA segments influenza A viruses (IAV) into viral particles is coordinated by segment-specific packaging sequences. How signals regulate specific incorporation each segment virions and whether other or host factors are involved in this process unknown. Here, we show that distinct amino acids nucleoprotein (NP) required for segments. This was determined studying NP a bat A-like virus, HL17NL10, context conventional IAV (SC35M). Replacement conserved...
The genome of influenza A virus is organized into eight viral ribonucleoproteins (vRNPs); this provides evolutionary advantages but complicates packaging. Although it has been shown that RNA packaging sequences and specific amino acids in the nucleoprotein (NP), both components each vRNP, ensure selective one copy vRNP per particle, required RNA-RNA RNA-NP interactions remain largely elusive. We identified mechanism tolerates mutation certain individual sequences, while their combined...
Abstract The genome of influenza A virus (IAV) consists eight unique viral RNA segments. This organization allows genetic reassortment between co-infecting IAV strains, whereby new IAVs with altered segment compositions emerge. While it is known that events can create pandemic IAVs, remains impossible to anticipate outcomes prospects. Recent research indicates promoted by a packaging mechanism delivers the segments as supramolecular complex into particle. finding holds promise predicting...
Efficient induction of interferon-stimulated genes (ISGs) prior to infection is known effectively convert a cell into an antiviral state, blocking viral replication. Additionally, cells can undergo caspase-mediated apoptosis control infection. Here, we identify SMARCA2 and SMARCA4 be essential for the efficient ISGs but also targeted by cellular caspases downstream intrinsic apoptotic pathway. We find that C-terminally cleaved accumulate at late stages infection, when damage already had...
ABSTRACT The coordinated packaging of the segmented genome influenza A virus (IAV) into virions is an essential step viral life cycle. This process controlled by interaction signals present in all eight RNA (vRNA) segments and nucleoprotein (NP), which binds vRNA via a positively charged binding groove. However, mechanistic models how NP work together to coordinate are missing. Here, we studied A/SC35M mutants that carry mutated as well specific amino acid substitutions at highly conserved...
Abstract A fundamental step in the influenza virus (IAV) replication cycle is coordinated packaging of eight distinct genomic RNA segments (i.e. vRNAs) into a viral particle. Although this process thought to be controlled by specific vRNA–vRNA interactions between genome segments, few functional have been validated. Recently, large number potentially detected purified virions using interactome capture method SPLASH. However, their significance remains largely unclear. Here, we show...
ABSTRACT The eight different genomic viral RNAs (vRNAs) of influenza A virus are typically packaged into virions as a (7 + 1) complex seven ribonucleoproteins (vRNPs) around central vRNP. Mutations in the packaging signals these vRNAs predicted to result lacking specific vRNPs. However, whether vRNPs then replaced by vRNP-like host is an open question. Here, we report that mutations two A/SC35M virus, which cause near-complete loss four from total virion population, several distinct...