A5005878847- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Erythropoietin and Anemia Treatment
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Cardiac Imaging and Diagnostics
- Advanced MRI Techniques and Applications
- DNA Repair Mechanisms
- Cardiovascular Disease and Adiposity
- Optical Imaging and Spectroscopy Techniques
- Advanced Fluorescence Microscopy Techniques
- Inhalation and Respiratory Drug Delivery
- Pneumonia and Respiratory Infections
- Cardiomyopathy and Myosin Studies
- Skin and Cellular Biology Research
- CRISPR and Genetic Engineering
- Occupational exposure and asthma
- bioluminescence and chemiluminescence research
- Photodynamic Therapy Research Studies
- Effects of Environmental Stressors on Livestock
- DNA and Nucleic Acid Chemistry
- Ultrasound and Hyperthermia Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Advanced biosensing and bioanalysis techniques
- Erythrocyte Function and Pathophysiology
- Genomics and Chromatin Dynamics
- Infrared Thermography in Medicine
PerkinElmer (United States)
2014-2024
South Ural State University
2019-2020
University of Groningen
2020
Haukeland University Hospital
2007
University of Bergen
2007
Montreal General Hospital
2002-2006
McGill University
2002-2006
Society for Arts and Technology
2005
The intratracheal instillation of bleomycin in mice induces early damage to alveolar epithelial cells and development inflammation followed by fibrotic tissue changes represents the most widely used model pulmonary fibrosis investigate human IPF. Histopathology is gold standard for assessing lung rodents, however it precludes repeated longitudinal measurements disease progression does not provide information on spatial temporal distribution damage. Here we investigated use Micro-CT technique...
Abstract Although increasing used in the preclinical testing of new anti-fibrotic drugs, a thorough validation micro-computed tomography (CT) pulmonary fibrosis models has not been performed. Moreover, no attempts have made so far to define density thresholds discriminate between aeration levels lung parenchyma. In present study, histogram-based analysis was performed mouse model bleomycin (BLM)-induced by micro-CT, evaluating longitudinal changes from 7 21 days after BLM challenge, period...
Abstract Recent studies suggest that erythropoietin plays an important role in the process of neoplastic transformation and malignant phenotype progression observed malignancy. To study its receptor (EPOR) on response cancer cells vitro, we used two solid tumor cell lines, namely human glioma line U87 primary cervical HT100. All experiments were done with heat-inactivated fetal bovine serum order to inactivate any endogenous erythropoietin. The expression EPOR these was confirmed immunoblot...
Human xenografts of acute myeloid leukemia (AML) in nonobese diabetic/severe combined immunodeficient (NOD/SCID) mice result disease states diffuse, nonpalpable tissue infiltrates exhibiting a variable course, with some animals not developing phenotype. Thus, staging and, more critically, quantification preclinical therapeutic effect these models are particularly difficult. In this study, we present the generation green fluorescent protein (GFP)-labeled human leukemic cell line, NB4, and...
The use of Micro-Computed Tomography (MicroCT) for in vivo studies small animals as models human disease has risen tremendously due to the fact that MicroCT provides quantitative high-resolution three-dimensional (3D) anatomical data non-destructively and longitudinally. Most importantly, with development a novel preclinical iodinated contrast agent called eXIA160, functional metabolic assessment heart became possible. However, prior advent commercial scanners equipped X-ray flat-panel...
The use of microscopic computed tomography to assess the key functional parameters systolic emptying or diastolic filling in small animals has not been previously reported. aim study was test whether can dynamics both left ventricle and right (RV) an experimental model pulmonary arterial hypertension Methods Results: Wistar-Kyoto rats were injected subcutaneously with VEGF (vascular endothelial growth factor)-receptor inhibitor SU5416 (20 mg/kg body weight) then exposed chronic hypoxia (10%...
Hypoxia, an important mechanism of radioresistance, is a strong stimulus for erythropoietin (EPO) production. The stimulatory effects EPO are mediated through the activation its receptors, receptors (EPORs). objective this study to determine whether EPORs expressed in biopsy specimens patients with squamous cell carcinoma cervix. Eighteen were studied after obtaining Institutional Review Board-approved consent. Standard immunohistochemistry techniques utilized. Expression was present 16 out...
Background: The intratracheal instillation of bleomycin in mice induces early damage to alveolar epithelial cells and development inflammation followed by fibrotic tissue changes represents the most widely used model pulmonary fibrosis investigate human IPF. Histopathology is gold standard for assessing lung rodents, however it precludes repeated longitudinal measurements disease progression does not provide information on spatial temporal distribution damage. Here we investigated use...
The use of Micro-Computed Tomography (MicroCT) for in vivo studies small animals as models human disease has risen tremendously due to the fact that MicroCT provides quantitative high-resolution three-dimensional (3D) anatomical data non-destructively and longitudinally. Most importantly, with development a novel preclinical iodinated contrast agent called eXIA160, functional metabolic assessment heart became possible. However, prior advent commercial scanners equipped X-ray flat-panel...
Micro-computed tomography derived functional biomarkers used in lung disease research can significantly complement end-stage histomorphometric measures while also allowing for longitudinal studies. However, no approach visualizing dynamics across a full respiratory cycle has yet been described. Using bleomycin-induced fibrosis and the antifibrotic drug nintedanib as test model, we implemented four-dimensional (4D) micro-CT imaging consisting of 30 reconstructed volumes per cycle, coupled...
<b>Introduction</b> Intratracheal bleomycin instillation in mice induces early damage to alveolar epithelial cells and development of inflammation followed by fibrotic tissue changes, mimicking human IPF. Even if histology is the gold standard quantify lung fibrosis mice, it precludes repeated longitudinal measurements disease progression does not provide information on 3D distribution damage. <b>Methods</b> We performed a comparative study bleomycin-induced C57Bl/6 using Mirco CT throughout...
Introduction and Hypothesis: The prevalence of myocardial infarction increases with the average age population is currently leading cause death worldwide. In order to define anatomical changes biomarkers related cardiac infarction, we tested use combined computed tomography (CT) near infrared fluorescent (NIRF) probes facilitate non-invasive imaging processes concerned tissue degeneration/regeneration. This development new diagnostic methods will lead better treatment options in future....
Introduction: Survival of patients with pulmonary hypertension (PH) is highly dependent on the right ventricular (RV) function. Despite association various factors adaptive or maladaptive RV phenotypes, contribution microvascular density to adaptation pressure overload remains unclear. Hypothesis: This study aimed evaluate myocardial blood volume and its role in overload. Methods: Both University Animal Care Committee federal authorities for animal research Regierungsprasidium Giessen...
Time domain imaging is presented as a method to perform in vivo fluorophore concentration reconstructions the presence of varying lifetimes. In particular, using SAMI/spl trade/platform, target and endogenous fluorescence are shown be distinguishable. Reconstruction solid Cy5.5 subcutaneous inclusion mouse done with background originating from food stomach. The discrimination between both sources by simple thereby providing better reconstruction.
Hypoxia, an important mechanism of radioresistance, is a strong stimulus for erythropoietin (EPO) production. The stimulatory effects EPO are mediated through the activation its receptors, receptors (EPORs). objective this study to determine whether EPORs expressed in biopsy specimens patients with squamous cell carcinoma cervix. Eighteen were studied after obtaining Institutional Review Board–approved consent. Standard immunohistochemistry techniques utilized. Expression was present 16 out...