A5043599907- Genomics and Phylogenetic Studies
- Genetic Mapping and Diversity in Plants and Animals
- Drug Transport and Resistance Mechanisms
- RNA and protein synthesis mechanisms
- Liver Disease Diagnosis and Treatment
- Chromosomal and Genetic Variations
- Plant Disease Resistance and Genetics
- Cholesterol and Lipid Metabolism
Huazhong Agricultural University
2023-2024
Shandong Provincial Hospital
2017
Shandong University
2017
Abstract Background Single-nucleotide polymorphisms (SNPs) are the most widely used form of molecular genetic variation studies. As reference genomes and resequencing data sets expand exponentially, tools must be in place to call SNPs at a similar pace. The genome analysis toolkit (GATK) is one SNP calling software publicly available, but unfortunately, high-performance computing versions this tool have yet become available affordable. Results Here we report an open-source variant workflow...
Abstract A high-performance computing genome variant calling workflow was designed to run GATK on HPC platforms. This efficiently called an average of 27.3 M, 32.6 168.9 and 16.2 M SNPs for rice, sorghum, maize, soybean, respectively, the most recently released high-quality reference sequences. Analysis a rice pan-genome panel revealed 2.1 novel that have yet be publicly released.
This study is aimed to develop and validate a high-performance liquid chromatography-tandem mass spectrometry method for the determination of bile acids in mouse serum. The serum samples were analysed after solid-phase extraction. analytes separated on Diamonsil C18 column with mobile phase methanol water containing 10 mM ammonium acetate 0.005% formic acid (70:30) at flow rate 0.5 ml/min. detected by tandem negative ion mode. results demonstrated that calibration curve was linear all over...