A5047826025- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Pluripotent Stem Cells Research
- RNA Interference and Gene Delivery
- Survey Methodology and Nonresponse
- Immune cells in cancer
- Neurofibromatosis and Schwannoma Cases
- Epigenetics and DNA Methylation
- Data Quality and Management
- Plant Virus Research Studies
- Adrenal and Paraganglionic Tumors
- Genomics and Phylogenetic Studies
- Cancer Cells and Metastasis
- Neuroblastoma Research and Treatments
- Signaling Pathways in Disease
- Census and Population Estimation
- Ion channel regulation and function
- Phosphodiesterase function and regulation
University of Michigan
2023
Michigan Medicine
2023
United States Census Bureau
2023
Harvard University
2016-2021
Boston University
2016
Significance Single-base substitutions are capable of producing transformative phenotypic changes. While methods to classify such mutations well established, it is difficult modulate or preclude their occurrence in a direct and efficacious manner. In this study, we refine the specificity CRISPR-Cas9 system present general framework for proactively preventing point mutations. This “mutation prevention system” broadly useful tool study control DNA substitutions, particularly contexts where an...
ABSTRACT Here we present a generalized method of guide RNA “tuning” that enables Cas9 to discriminate between two target sites differ by single nucleotide polymorphism. We employ our methodology generate novel in vivo mutation prevention system which actively restricts the occurrence undesired gain-of-function mutations within population engineered organisms. further demonstrate is scalable multitude targets and general tuning concepts are portable across variants orthologs. Finally, show...
ABSTRACT Construction of genetic variant libraries with phenotypic measurement is central to advancing today’s functional genomics, and remains a grand challenge. Here, we introduce Cas9-based approach for generating pools mutants defined alterations (deletions, substitutions insertions), along methods tracking their fitness en masse . We demonstrate the utility our in performing focused analysis hundreds single protein investigating biological function an entire family poorly characterized...
The U.S. Census Bureau conducts a census of population and housing every 10 years as mandated in the Constitution. Following up person with households that do not respond online, by phone, or mail, which is known nonresponse follow-up (NRFU), represents major component this effort. For 2010 Census, equipped enumerators paper maps notebooks filled questionnaires required to go door collect decennial data. met daily their supervisors return completed update payroll information. 2020 an...
Abstract CRISPR/Cas9 has revolutionized the field of genome engineering. Yet, as CRISPR toolbox rapidly expanded, there remains a need for comprehensive library reagents that allow users to perform complex cellular and genetic manipulations without requiring labor-intensive generation meet each user’s unique experimental circumstances. Here we described creation validation pNAX consisting 72 different Cas9 gRNA expression plasmids efficient multiplex gene editing, activation, repression in...
Ganglioneuromas are benign tumors arising from the neural crest. Histologically, they composed of mature Schwann cells and ganglion admixed with fibrous tissue. While frequently seen in abdomen mediastinum, rare reports have highlighted their occurrences genitourinary system. The only prior reported prostatic ganglioneuroma arose a patient history neurofibromatosis type 1. In this report, we highlight first without known genetic linkage.