A5000471538- Cell Adhesion Molecules Research
- Cellular Mechanics and Interactions
- Biochemical and Structural Characterization
- Autophagy in Disease and Therapy
- Phagocytosis and Immune Regulation
- Calcium signaling and nucleotide metabolism
- Extracellular vesicles in disease
- Cellular transport and secretion
University of Groningen
2022-2023
In autophagy, LC3‐positive autophagophores fuse and encapsulate the autophagic cargo in a double‐membrane structure. contrast, lipidated LC3 (LC3‐II) is directly formed at phagosomal membrane LC3‐associated phagocytosis (LAP). this study, we dissected effects of autophagy inhibitors on LAP. SAR405, an inhibitor VPS34, reduced levels LC3‐II inhibited endosomal acidification bafilomycin A1 chloroquine increased LC3‐II, due to degradation acidic lysosomes. However, while LAP, did not. Finally,...
Endosomal Sorting Complex Required for Transport (ESCRT) proteins can be transiently recruited to the plasma membrane repair and formation of extracellular vesicles. Here, we discovered micrometer-sized worm-shaped ESCRT structures that stably persist multiple hours at macrophages, dendritic cells, fibroblasts. These surround clusters integrins known cargoes The are tightly connected cellular support left behind by cells together with surrounding patches membrane. phospholipid composition is...