A5103126391- Neuropeptides and Animal Physiology
- Neurobiology and Insect Physiology Research
- Pain Mechanisms and Treatments
- Neuroscience and Neuropharmacology Research
- Dermatology and Skin Diseases
- Neural dynamics and brain function
- Neuroscience and Neural Engineering
- Stress Responses and Cortisol
- Hypothalamic control of reproductive hormones
- Pharmacological Receptor Mechanisms and Effects
- Ion Channels and Receptors
- Neuroscience of respiration and sleep
- Pain Management and Placebo Effect
- Urticaria and Related Conditions
- Axon Guidance and Neuronal Signaling
University of Glasgow
2021-2025
Biotechnologie et Signalisation Cellulaire
2018
Université de Strasbourg
2018
Centre National de la Recherche Scientifique
2018
The anterolateral system (ALS) is a major ascending pathway from the spinal cord that projects to multiple brain areas and underlies perception of pain, itch, skin temperature. Despite its importance, our understanding this has been hampered by considerable functional molecular diversity constituent cells. Here, we use fluorescence-activated cell sorting isolate ALS neurons belonging Phox2a-lineage for single-nucleus RNA sequencing. We reveal five distinct clusters (ALS1-5) document their...
Abstract Neurons in the superficial dorsal horn that express gastrin-releasing peptide receptor (GRPR) are strongly implicated spinal itch pathways. However, a recent study reported many of these correspond to vertical cells, population interneurons believed transmit nociceptive information. In this study, we have used GRPR CreERT2 mouse line identify and target cells possess Grpr mRNA. We find highly concentrated lamina I outer part II, they all glutamatergic, account for ∼15% excitatory...
Abstract Anterolateral system (ALS) spinal projection neurons are essential for pain perception. However, these cells heterogeneous, and there has been extensive debate about the roles of ALS populations in different dimensions. We recently performed single-nucleus RNA sequencing on a developmentally-defined subset neurons, identified 5 transcriptomic populations. One these, ALS4, consists that express Sst , gene coding somatostatin, we reported were located lateral part lamina V. Here use...
Anterolateral system (ALS) projection neurons underlie perception of pain, itch and skin temperature. These cells are heterogeneous, there have therefore been many attempts to define functional populations. A recent study identified two classes ALS neuron in mouse superficial dorsal horn (SDH) based on expression the G protein-coupled receptors Tacr1 or Gpr83. It was reported that expressing these formed largely non-overlapping populations, ~60% SDH expressed Tacr1. An additional finding...
Anterolateral system (ALS) projection neurons underlie perception of pain, itch and skin temperature. These cells are heterogeneous, there have therefore been many attempts to define functional populations. A recent study identified two classes ALS neuron in mouse superficial dorsal horn (SDH) based on expression the G protein-coupled receptors Tacr1 or Gpr83. It was reported that expressing these formed largely non-overlapping populations, ~60% SDH expressed Tacr1. An additional finding...
Abstract Excitatory interneurons in the superficial dorsal horn (SDH) are heterogeneous, and include a class known as vertical cells, which convey information to lamina I projection neurons. We recently used pro-NPFF antibody reveal discrete population of excitatory that express neuropeptide FF (NPFF). Here, we generated new mouse line (NPFF Cre ) is knocked into Npff locus, Cre-dependent viruses reporter mice characterise NPFF cell properties. Both viral strategies labelled many cells SDH,...
Abstract A recently developed Phox2a::Cre mouse line has been shown to capture anterolateral system (ALS) projection neurons. Here, we used this test whether Phox2a-positive cells represent a distinct subpopulation among lamina I ALS We show that virtually all Phox2a can be retrogradely labelled from injections targeted on the lateral parabrachial area (LPb), and most of those in cervical cord also belong spinothalamic tract. accounted for ~ 50–60% LPb or thalamus. was preferentially...
Abstract The anterolateral system (ALS) is a major ascending pathway from the spinal cord that projects to multiple brain areas and underlies perception of pain, itch skin temperature. Despite its importance, our understanding this has been hampered by considerable functional molecular diversity constituent cells. Here we use fluorescence-activated cell sorting isolate ALS neurons belonging Phox2a-lineage for single-nucleus RNA sequencing. We reveal five distinct clusters (ALS1-5) document...
The superficial dorsal horn (SDH) of the spinal cord contains a diverse array neurons. vast majority these are interneurons, most which glutamatergic. These can be assigned to several populations, one is defined by expression gastrin-releasing peptide receptor (GRPR). GRPR cells thought "tertiary pruritoceptors," conveying itch information lamina I projection neurons anterolateral system (ALS). Surprisingly, we recently found that GRPR-expressing belong morphological class known as vertical...
Anterolateral system (ALS) spinal projection neurons are essential for pain perception. However, these cells heterogeneous, and there has been extensive debate about the roles of ALS populations in different dimensions. We recently performed single-nucleus RNA sequencing on a developmentally-defined subset neurons, identified 5 transcriptomic populations. One these, ALS4, consists that express Sst, gene coding somatostatin, we reported were located lateral part lamina V. Here use SstCre...
Gastrin-releasing peptide (GRP) in the spinal dorsal horn acts on GRP receptor, and this signalling mechanism has been strongly implicated itch. However, source of is not fully understood. For example, BAC transgenic mouse line GRP::GFP only captures around 25% GRP-expressing cells, Grp mRNA found several types excitatory interneuron. A major limitation attempts to identify neurons that antibodies against cross-react with other neuropeptides, including some are expressed by primary...