We present data showing that some foods preserved in lacquer-coated cans and the liquid them may acquire estrogenic activity. Hormonal activity was measured using E-screen bioassay. The biological of vegetables packed a result plastic monomers used manufacturing containers. monomer bisphenol-A, identified by mass spectrometry, found as contaminant not only but also water autoclaved cans. amount bisphenol-A extracts accounted for all hormonal measured. Although presence other xenoestrogens...

MCF7 human breast cancer cells have been studied extensively as a model for hormonal effects on cell growth and specific protein synthesis. Because the proliferative effect of natural estrogen is considered hallmark action, it was proposed that this property be used to determine whether substance an estrogen. The E-screen assay, developed purpose, based ability proliferate in presence estrogens. aim our study characterize response four stocks (BUS, ATCC, BB, BB104) which them performed best...

The chemical structure of hydroxylated diphenylalkanes or bisphenols consists two phenolic rings joined together through a bridging carbon. This class endocrine disruptors that mimic estrogens is widely used in industry, particularly plastics. Bisphenol F, bisphenol A, fluorine-containing A (bisphenol AF), and other were found to be estrogenic bioassay with MCF7 human breast cancer cells culture (E-SCREEN assay). Bisphenols promoted cell proliferation increased the synthesis secretion...

We present data showing that some foods preserved in lacquer-coated cans and the liquid them may acquire estrogenic activity. Hormonal activity was measured using E-screen bioassay. The biological of vegetables packed a result plastic monomers used manufacturing containers. monomer bisphenol-A, identified by mass spectrometry, found as contaminant not only but also water autoclaved cans. amount bisphenol-A extracts accounted for all hormonal measured. Although presence other xenoestrogens...

To evaluate the relevance of ongoing nociceptive joint inputs to maintenance widespread pain hypersensitivity in patients with hip osteoarthritis (OA) and determine whether a reversal pressure together an improvement function occurs after total replacement these patients.Forty OA participated. Twenty underwent replacement, other 20 were assigned waiting list. Pressure-pain thresholds (PPTs) over second metacarpal bone gluteus medius, vastus medialis, lateralis, tibialis anterior muscles...

AbstractThe aim of the present study was to investigate association between gene hypermethylation and main clinicopathological features breast cancer, including diagnosis treatment response. A sensitive SYBR green methylation-specific PCR technique used analyze utility circulating DNA with CpG island ESR1, APC, RARB, 14-3-3-σ, E-cad promoter regions as cancer biomarkers. Analyses were conducted preoperative sera from 106 women 34 benign disease 74 no evidence post-treatment 60 patients. Mean...

Abstract Introduction Radiotherapy outcomes might be further improved by a greater understanding of the individual variations in normal tissue reactions that determine tolerance. Most published studies on radiation toxicity have been performed retrospectively. Our prospective study was launched 1996 to measure vitro radiosensitivity peripheral blood lymphocytes before treatment with radical radiotherapy patients breast cancer, and assess early late skin side effects same group patients. We...

Pinar, B., Lara, P. C., Lloret, M., Bordón, E., Núñez, M. I., Villalobos, Guerrero, R., Luna, J. D. and Ruiz de Almodóvar, Radiation-Induced DNA Damage as a Predictor of Long-Term Toxicity in Locally Advanced Breast Cancer Patients Treated with High-Dose Hyperfractionated Radical Radiotherapy. Radiat. Res. 168, 415–422 (2007).This 14-year-long study makes novel contribution to the debate on relationship between vitro radiosensitivity peripheral blood lymphocytes normal tissue reactions after...

The success of radiotherapy in eradicating tumours depends on the total radiation dose, but what limits this dose is tolerance normal tissues within treatment volume. Studies involving fibroblast survival have demonstrated theoretical feasibility a predictive assay sensitivity, such an still far from clinical application. Using pulsed-field gel electrophoresis (PFGE), we quantified initial "apparent" number DNA double-strand breaks (dsb) induced by as alternative measure sensitivity 2...

. Treatments which inhibit or retard progression of the cell through cycle have been reported to reduce effectiveness ionizing radiation by increasing cellular radioresistance. We studied radiosensitivity and radiation-induced DNA damage (double-strand break, dsb) in both hormone-sensitive non-sensitive human breast cancer lines. After 72 h culture an oestradioldeprived medium, MCF-7 BUS T47D B8 cells showed a significant delay growth, whereas no effect was seen EVSA-Tcell line. In...

We propose and study a new model aimed at describing the low-dose hyper-radiosensitivity phenomenon appearing in survival curves of different cell lines.The uses induced repair assumption, considering that critical dose which this mechanism begins to act varies from given population. The proposed is compared with linear-quadratic modified model, commonly used literature taken into account heuristic way. curve for MCF-7 line human breast cancer measured low absorbed doses uncertainties these...

MCF7 human breast cancer cells have been studied extensively as a model for hormonal effects on cell growth and specific protein synthesis. Because the proliferative effect of natural estrogen is considered hallmark action, it was proposed that this property be used to determine whether substance an estrogen. The E-screen assay, developed purpose, based ability proliferate in presence estrogens. aim our study characterize response four stocks (BUS, ATCC, BB, BB104) which them performed best...

Abstract. Growth data on 60 multicellular spheroids of MCF‐7 human breast cancer cells were fitted, an individual basis, by the Gompertz, Bertalanffy and logistic equations. spheroids, initiated grown in medium containing oestrogens, exhibited a growth rate that decreased continuously as spheroid size increased. Plots volume v. time generated sigmoid curves showed early portion with approximately exponential increase; middle region or retardation phase characterized decreasing rate; and,...

MCF7 human breast cancer cells growing as multicellular spheroids were examined a model of three-dimensional cellular organization. Estrogen-free medium inhibited spheroid formation. In containing estrogens, the antiestrogen hydroxytamoxifen decreased growth rate. Analyses with recursion formula after Gompertz fitting showed that rate exponential decrease in (alpha) was alpha 0.099 +/- 0.013 d-1, and alpha' 0.061 0.015 d-1 for 0.1 microM control respectively. which had been arrested an...

Abstract To obtain multicellular spheroids from MCF‐7 human breast cancer cells we adhered to the following procedure: (a) limiting adherence of cell substratum; (b) seeding more than minimum number cells; (c) guaranteeing presence estrogens in culture medium. Charcoal‐dextran (CO)‐ treated sera seemed inhibit spheroid formation. A reduction concentration CD‐human (from 10% 5%) added phenol‐red‐free medium facilitated progress cellular aggregates spheroids. Once became initiated, size...

To assess the potential relationship between p53 and p16 proteins in cellular response to stress, we have examined levels of these a series human tumor cell lines after treatment with either ionizing radiation or hyperthermia. We found that cells abnormal radiation-induced G1 arrest (non-functional p53) had significantly higher constitutive than showing normal (functional p53). Time-course experiments were done test effect γ-irradiation on intracellular p16. The pattern changes was similar...

The chemical structure of hydroxylated diphenylalkanes or bisphenols consists two phenolic rings joined together through a bridging carbon. This class endocrine disruptors that mimic estrogens is widely used in industry, particularly plastics. Bisphenol F, bisphenol A, fluorine-containing A (bisphenol AF), and other were found to be estrogenic bioassay with MCF7 human breast cancer cells culture (E-SCREEN assay). Bisphenols promoted cell proliferation increased the synthesis secretion...