A5013691177- Medical Imaging Techniques and Applications
- Peptidase Inhibition and Analysis
- Click Chemistry and Applications
- Synthesis and Catalytic Reactions
- Signaling Pathways in Disease
- Galectins and Cancer Biology
- Fibroblast Growth Factor Research
- Advanced Fluorescence Microscopy Techniques
- Aquaculture Nutrition and Growth
- Enzyme Production and Characterization
- Atomic and Subatomic Physics Research
- Genomics and Phylogenetic Studies
- Sphingolipid Metabolism and Signaling
- Microbial Natural Products and Biosynthesis
- Protein Hydrolysis and Bioactive Peptides
- Advanced Biosensing Techniques and Applications
- Atherosclerosis and Cardiovascular Diseases
- Characterization and Applications of Magnetic Nanoparticles
- Reproductive biology and impacts on aquatic species
- Radiation Dose and Imaging
- Tracheal and airway disorders
- Neonatal Respiratory Health Research
- Sulfur-Based Synthesis Techniques
- Computational Drug Discovery Methods
- Cholesterol and Lipid Metabolism
Scripps (United States)
2024
Scripps Institution of Oceanography
2024
Scripps Research Institute
2023-2024
Memorial Sloan Kettering Cancer Center
2022
The University of Texas at Austin
2019
Type 2 alveolar epithelial cells (AEC2s) are stem in the adult lung that contribute to lower airway repair. Agents promote selective expansion of these might stimulate regeneration compromised epithelium, an etiology-defining event several pulmonary diseases. From a high-content imaging screen drug repurposing library ReFRAME, we identified dipeptidyl peptidase 4 (DPP4) inhibitors, widely used type diabetes medications, selectively expand AEC2s and broadly efficacious mouse models damage....
The proteins of trans-acyltransferase modular polyketide synthases (PKSs) self-organize into assembly lines, enabling the multienzyme biosynthesis complex organic molecules. Docking domains comprised ∼25 residues at C- and N-termini these polypeptides (CDDs NDDs) help drive this association through formation four-helix bundles. Molecular connectors like are desired in synthetic contexts, such as artificial biocatalytic systems biomaterials, to orthogonally join proteins. Here, ability six...
Abstract A central regulator of metabolism, transcription factor carbohydrate response element binding protein (ChREBP) senses and responds to dietary glucose levels by stimulating the glycolytic lipogenic enzymes. Genetic depletion ChREBP rescues β-cell dysfunction arising from high levels, suggesting that inhibiting might represent an attractive therapeutic approach manage diabetes other metabolic diseases. However, molecular mechanisms governing activation are poorly understood chemical...
Small molecule dyes remain essential biological tools, yet only a handful of environmentally responsive fluorogenic small molecules are available for routine characterization protein state. Here, we report the development and execution high throughput screen to identify compounds that increase in fluorescence response binding lipophilic sites proteins. This effort yielded two potently indicate presence range common proteins outperform differential scanning fluorimetry experiments. Structure...
Cysteine reactive groups are a mainstay in the design of covalent drugs and probe molecules, yet only handful electrophiles routinely used to target this amino acid. Here, we report development scalable thiol reactivity (STRP), method which enables facile interrogation large chemical libraries for intrinsic with cysteine. High throughput screening using STRP identified azetidinyl oxadiazole as moiety that selectively reacts cysteine through ring opening-based mechanism, capable covalently...