A5068541414- Monoclonal and Polyclonal Antibodies Research
- Protein Structure and Dynamics
- HER2/EGFR in Cancer Research
- Machine Learning in Bioinformatics
- T-cell and B-cell Immunology
- Glycosylation and Glycoproteins Research
- Genomics and Phylogenetic Studies
- Cancer Immunotherapy and Biomarkers
- Advanced Biosensing Techniques and Applications
- Enzyme Structure and Function
- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
- Genetics, Bioinformatics, and Biomedical Research
- Bioinformatics and Genomic Networks
- Biosimilars and Bioanalytical Methods
- CAR-T cell therapy research
- RNA and protein synthesis mechanisms
- Chronic Lymphocytic Leukemia Research
- Endoplasmic Reticulum Stress and Disease
- Lipid Membrane Structure and Behavior
- Immune Cell Function and Interaction
- Immunodeficiency and Autoimmune Disorders
- Biomedical Text Mining and Ontologies
- Fungal and yeast genetics research
- Computational Drug Discovery Methods
Elbit Systems (Israel)
2018-2024
Bar-Ilan University
2010-2024
Tel Aviv University
2006-2013
Hungarian Academy of Sciences
2010
Memorial Sloan Kettering Cancer Center
2010
Institute of Molecular Life Sciences
2010
Abstract Many mutations disappear from the population because they impair protein function and/or stability. Thus, amino acid positions that are essential for proper evolve more slowly than others, or in other words, slow evolutionary rate of a position reflects its importance. ConSurf ( http://consurf.tau.ac.il ), reviewed this manuscript, exploits to reveal key important maintaining native conformation(s) and function, be it binding, catalysis, transport, etc. Given sequence 3D structure...
ConSurf-DB is a repository for evolutionary conservation analysis of the proteins known structures in Protein Data Bank (PDB). Sequence homologues each PDB entries were collected and aligned using standard methods. The amino acid position alignment was calculated Rate4Site algorithm, implemented ConSurf web server. algorithm takes into account phylogenetic relations between stochastic nature process explicitly. assigns level multiple sequence an empirical Bayesian inference. Visual...
The ultimate goal of protein design is to introduce new biological activity. We propose a computational approach for designing functional antibodies by focusing on epitopes, integrating large-scale statistical analysis with multiple structural models. Machine learning used analyze these models and predict specific residue-residue contacts. use this antibody counter the proinflammatory effect cytokine interleukin-17A (IL-17A). X-ray crystallography confirms that designed binds targeted...
The ECM33/SPS2 family of glycosylphosphatidylinositol-anchored proteins plays an important role in maintaining fungal cell wall integrity and virulence. However, the precise molecular these is unknown. In this work, AfuEcm33, gene encoding ECM33 homologue pathogenic fungus Aspergillus fumigatus, has been cloned its function analysed. It shown that disruption AfuEcm33 results rapid conidial germination, increased cell-cell adhesion, resistance to antifungal agent caspofungin virulence...
Abstract Summary: The iDBPs server uses the three-dimensional (3D) structure of a query protein to predict whether it binds DNA. First, algorithm predicts functional region based on its evolutionary profile; assumption is that large clusters conserved residues are good markers regions. Next, various characteristics predicted as well global features calculated, such average surface electrostatic potential, dipole moment and cluster-based amino acid conservation patterns. Finally, random...
Deep parallel sequencing (NGS) is a viable tool for monitoring scFv and Fab library dynamics in many antibody engineering high-throughput screening efforts. Although very useful, the commonly used Illumina NGS platform cannot handle entire sequence of or single read, usually focusing on specific CDRs resorting to VH VL variable domains separately, thus limiting its utility comprehensive selection dynamics. Here we present simple robust method deep repertoires full length scFv, Fv sequences....
Stimulating effector T-cells (Teffs) without inducing regulatory (Tregs) has been the primary goal of IL-2-based therapies for cancer. Recently, modified IL-2 designed differential T-cell expansion treatment cancer failed in clinic. We propose that treatments based on exogenous administrations are inherently undermined by a negative feedback loop, caused secreted endogenously from activated T-cells. This endogenous secretion subsequentially induces Treg and inhibits immune response is...
<h3>Background</h3> IL-2 binds two forms of receptor: a high affinity trimeric receptor composed CD25, CD122, and CD132, low dimeric CD122 CD132. Binding to the receptors, expressed on effector cells, causes expansion arm immune system including CD8 T-cells, NK-cells NKT-cells. receptor, Tregs as well pulmonary vascular epithelium, results in Treg cells Vascular Leak Syndrome, both are undesired outcomes high-dose recombinant (Aldesleukin), approved for treatment Melanoma...
Genomic germline and somatic variations may impact drug binding even lead to resistance. However, designing a different for each mutant not be feasible. In this study, we identified the most common cancer mutations from Catalogue of Somatic Mutations in Cancer (COSMIC) that occur structurally characterized sites approved therapeutic antibodies. We found two HER2 mutations, S310Y S310F, substantially compromise Pertuzumab, widely used therapeutics, To address these designed multi-specific...
Abstract Antibody engineering technology is at the forefront of therapeutic antibody development. The primary goal for a generation an with desired specificity, affinity, function, and developability profile. Mature antibodies are considered antigen specific, which may preclude their use as starting point engineering. Here, we explore plasticity mature by novel specificity function to pre-selected template. Using small, focused library, engineered AAL160, anti-IL-1β antibody, bind unrelated...
Genomic germline and somatic variations may impact drug binding even lead to resistance. However, designing a different for each mutant not be feasible. In this study, we identified the most common cancer mutations from Catalogue of Somatic Mutations in Cancer (COSMIC) that occur structurally characterized sites approved therapeutic antibodies. We found two HER2 mutations, S310Y S310F, substantially compromise Pertuzumab, widely used therapeutics, To address these designed multi-specific...
Genomic germline and somatic variations may impact drug binding even lead to resistance. However, designing a different for each mutant not be feasible. In this study, we identified the most common cancer mutations from Catalogue of Somatic Mutations in Cancer (COSMIC) that occur structurally characterized sites approved therapeutic antibodies. We found two HER2 mutations, S310Y S310F, substantially compromise Pertuzumab, widely used therapeutics, To address these designed multi-specific...
Abstract Genomic germline and somatic variations may impact drug binding even lead to resistance. However, designing a different for each mutant not be feasible. In this study, we identified the most common cancer mutations from Catalogue of Somatic Mutations in Cancer (COSMIC) that occur structurally characterized sites approved therapeutic antibodies. We found two HER2 mutations, S310Y S310F, substantially compromise Pertuzumab, widely used therapeutics, To address these designed...
e15004 Background: Interleukin 2 is a 15.4 kDa type I cytokine of the four helix bundle structure. IL-2 signaling has two opposite effects, it can enhance immune response by activation effector cells and regulate proliferation regulatory T (Tregs). mediates its effect binding to forms receptor: i) trimeric receptors made IL-2Rα (CD25), IL-2Rβ (CD122), common IL-2Rγ (γc, CD132) chains or ii) dimeric receptor only subunits. In Tregs, associated with FoxP3 mediated transcription leading...