Serum Autoantibodies to Optic Nerve Head Glycosaminoglycans in Patients With Glaucoma

• DOI: 10.1001/archopht.117.7.917
• Publication Date: 2012-06-05T06:14:42Z
• Authors: Gülgün Tezel
• Keywords: Optic nerve diseases

<h3>Background</h3> Serum autoantibodies that cross-react with glycosaminoglycans have been proposed to play a significant role in specific tissue injury patients systemic autoimmune diseases. <h3>Objective</h3> To investigate whether serum immunoreactivity is present glaucoma who aberrant DNA, RNA, nuclear proteins, or retinal as proteoglycans and their glycosaminoglycan side chains are important components of the optic nerve head its vasculature. <h3>Methods</h3> We performed Western blotting using patient samples human homogenates were treated without degrading enzymes. Monoclonal antibodies recognize different determinants used identify substrate antigenicity. compared 60 age-matched normal-pressure glaucoma, 36 primary open-angle 20 control subjects by enzyme-linked immunosorbent assay. In addition, immunohistochemistry was compare distribution patterns postmortem eyes subjects. <h3>Results</h3> demonstrated from circulating can proteoglycans, including chondroitin sulfate heparan sulfate. The level binding purified an assay approximately 100% higher than 50% glaucoma. also observed increased immunostaining particularly those normal intraocular pressure, eyes. <h3>Conclusion</h3> There levels recognizing some <h3>Clinical Relevance</h3> These may increase susceptibility damage these changing functional properties lamina cribrosa, vasculature, both.