Long noncoding RNA LINC01578 drives colon cancer metastasis through a positive feedback loop with the NF‐κB/YY1 axis

• DOI: 10.1002/1878-0261.12819
• Publication Date: 2020-10-11T04:13:58Z
• Authors: Jia Liu; Yang Zhan; Jiefu Wang; Junfeng Wang; Jiansheng Guo; Dalu Kong
• Keywords: Feedback, Physiological; Male; 0301 basic medicine; Mice, Inbred BALB C; Base Sequence; Cell Survival; Liver Neoplasms; NF-kappa B; Mice, Nude; Middle Aged; Epigenesis, Genetic; 3. Good health; Gene Expression Regulation, Neoplastic; 03 medical and health sciences; Cell Movement; Cell Line, Tumor; Colonic Neoplasms; Animals; Humans; Enhancer of Zeste Homolog 2 Protein; Female; I-kappa B Proteins; Neoplasm Metastasis; Research Articles

Metastasis accounts for poor prognosis of cancers and related deaths. Accumulating evidence has shown that long noncoding RNAs (lncRNAs) play critical roles in several types cancer. However, which lncRNAs contribute to metastasis colon cancer is still largely unknown. In this study, we found lncRNA LINC01578 was correlated with upregulated cancer, associated metastasis, advanced clinical stages, overall survival, disease-specific disease-free survival. Gain-of-function loss-of-function assays revealed enhanced cell viability mobility vitro liver vivo. Mechanistically, nuclear factor kappa B (NF-κB) Yin Yang 1 (YY1) directly bound the promoter, its activity, activated expression. be a chromatin-bound lncRNA, NFKBIB promoter. Furthermore, interacted recruited EZH2 promoter further repressed expression, thereby activating NF-κB signaling. Through activation NF-κB, YY1 NF-κB/YY1 axis, turn own suggesting formed positive feedback loop. Blocking signaling abolished oncogenic expression levels LINC01578, NFKBIB, were tissues. Collectively, study demonstrated promoted via forming loop suggested represents potential prognostic biomarker therapeutic target metastasis.