Amnion Epithelial Cell Isolation and Characterization for Clinical Use

• DOI: 10.1002/9780470151808.sc01e06s13
• Publication Date: 2010-04-07T04:49:17Z
• Authors: Sean Murphy; Sharina Rosli; Rutu Acharya; Louisa Mathias; Rebecca Lim; Euan Wallace; Graham Jenkin
• Keywords: Amnion; Germ layer; Amniotic epithelial cells

Abstract Human amnion epithelial cells (hAECs) are a heterologous population positive for stem cell markers; they display multilineage differentiation potential, differentiating into of the endoderm (liver, lung epithelium), mesoderm (bone, fat), and ectoderm (neural cells). They have low immunogenic profile possess potent immunosuppressive properties. Hence, hAECs may be valuable source therapy. This unit describes an efficient effective method hAEC isolation, culture, cryopreservation that is animal product–free in accordance with current guidelines on preparation clinical use. Cells isolated using this were characterized after 5 passages by analysis karyotype, cycle distribution, changes telomere length. The potential was demonstrated lineages three primary germ layers expression lineage‐specific markers analyzed PCR, immunocytochemistry, histology. Curr. Protoc. Stem Cell Biol . 13:1E.6.1‐1E.6.25. © 2010 John Wiley & Sons, Inc.