Differential Impact of Fluid Shear Stress and YAP/TAZ on BMP/TGF‐β Induced Osteogenic Target Genes

• DOI: 10.1002/adbi.202000051
• Publication Date: 2021-01-15T22:28:27Z
• Authors: Maria Reichenbach; Paul‐Lennard Mendez; Carolina da Silva...; Vladimir Ugorets; Paul Rikeit; Stefan Boerno; Jerome Jatzlau; Petra Knaus
• Keywords: Crosstalk

Bone is a remarkable dynamic structure, which integrates mechanical and biochemical signaling inputs. Interstitial fluid in the intramedullary space transmits signals derived from compression-induced shear stress (FSS) to stimulate osteoblasts for bone formation. Using flow system human osteoblasts, this study demonstrates how BMP/TGF-β stimuli FSS YAP/TAZ confirms these findings by transcriptome analyses. Here, positively affects phosphorylation of both SMAD1/5 SMAD2/3, respective BMP- TGFβ-R-SMADs. Increase phosphorylated levels distinct target genes, are susceptible low SMADs (such as ID1-3) or dependent on high (NOG, noggin). Thus, lowers threshold genes when less BMP available. While impact direct independent YAP/TAZ, acts cooperatively with TGF-β shared pathways CTGF). As key regeneration, their crosstalk such different levels, allows now think about new more specified intervention strategies age-related loss.