Modeling Foam Cell Formation in A Hydrogel‐Based 3D‐Intimal Model: A Study of The Role of Multi‐Diseases During Early Atherosclerosis

• DOI: 10.1002/adbi.202300463
• Publication Date: 2024-01-11T05:14:50Z
• Authors: Fahima Akther; Dimple Sajin; Shehzahdi S. Moonshi; Yuao Wu; Karla X Vazquez‐P...; Hang Thu Ta
• Keywords: Monocyte; Foam cell

Monocyte recruitment and transmigration are crucial in atherosclerotic plaque development. The multi-disease complexities aggravate the situation continue to be a constant concern for understanding atherosclerosis Herein, 3D hydrogel-based model that integrates disease-induced microenvironments is sought designed, allowing us explore early stages of atherosclerosis, specifically examining monocyte fate complexities. As proof-of-concept study, murine cells employed develop model. constructed with collagen embedded aortic smooth muscle endothelial monolayer lining. achieves vitro disease using external stimuli such as glucose lipopolysaccharide (LPS). Hyperglycemia exhibits significant increase adhesion but no enhancement foam cell conversion compared euglycemia. Chronic infection achieved by LPS stimulation results remarkable augment initial attachment increment all concentrations. Moreover, synergistic sensitivity under conditions hyperglycemia infection, enhancing attachment, transmigration, formation. Additionally, western blot data prove enhanced levels inflammatory biomarkers, indicating model's capability mimic during progression.