Polymer Electrochromism Driven by Metabolic Activity Facilitates Rapid and Facile Bacterial Detection and Susceptibility Evaluation

• DOI: 10.1002/adfm.202005192
• Publication Date: 2020-09-24T12:02:05Z
• Authors: Jiayingzi Wu; Yifan Zhu; Liyan You; Pu‐Ting Dong; Jianguo Mei; Ji‐Xin Cheng
• Keywords: Gram-Negative Bacteria

Abstract The electrochromism of a water‐soluble naturally oxidized electrochromic polymer, ox‐PPE, is harnessed for rapid and facile bacterial detection, discrimination, susceptibility testing. ox‐PPE solution shows distinct colorimetric spectroscopic changes within 30 min when mixed with live bacteria. For the underlying mechanism, it found that responds to reducing species (e.g., cysteine glutathione) released by metabolically active This reduction reaction ubiquitous among various strains, noticeable difference enables discrimination Gram‐negative Gram‐positive strains. Combining antibiotics, methicillin‐susceptible methicillin‐resistant Staphylococcus aureus can be differentiated 2.5 h. Proof‐of‐concept demonstration antimicrobial testing carried out incubating Escherichia coli antibiotics. obtained minimum inhibition concentrations are consistent conventional culture‐based methods, but procedure time significantly shortened 3