Bone Microenvironment‐Mimetic Scaffolds with Hierarchical Microstructure for Enhanced Vascularization and Bone Regeneration

• DOI: 10.1002/adfm.202200011
• Publication Date: 2022-02-09T11:10:02Z
• Authors: Yujie Ha; Xiaojun Ma; Shikai Li; Tao Li; Zhihui Li; Yuhan Qian; Muhammad Shafiq; Jinwu Wang; Xiaojun Zhou; Chuanglong He
• Keywords: 02 engineering and technology; 0210 nano-technology

Abstract Microchannel networks within engineered 3D scaffold can allow nutrient exchange and rapid blood vessels formation. However, fabrication of a bone microenvironment‐mimicking with hierarchical micro/nanofibrous microchannel structures is still challenge. Herein, inspired by structural functional cues remodeling, networks‐enriched nanofibrous using printing thermally induced phase separation techniques, which facilitate cells migration nutrients transportation, developed. The customizable vascular‐like structure polycaprolactone the gelatin‐silica fabricated 3D‐printed sacrificial templates, while dimethyloxalylglycine (DMOG)‐loaded mesoporous silica nanoparticles (MSNs) located on surface forming peptide‐1 (BFP)‐loaded MSNs embedded in are implemented for sequential release DMOG BFP. cell experiments show that dual‐drug delivery (DBM/GP) promotes angiogenesis stimulating migration, tube formation, angiogenesis‐related genes/protein expression endothelial cells, osteogenesis promoting osteo‐related genes mineral deposition osteoblasts. Additionally, DBM/GP facilitates angiogenic activity osteoblasts activating phosphatidylinositol 3‐kinase/protein kinase B/hypoxia inducible factor‐1α pathway. Furthermore, enhanced vascularization regeneration demonstrated via subcutaneous skull defect models. Overall, this study reveals microenvironment‐mimetic provides promising strategy defects treatment.