Co‐Encapsulating Enzymes and Carbon Dots in Metal–Organic Frameworks for Highly Stable and Sensitive Touch‐Based Sweat Sensors

• DOI: 10.1002/adfm.202310121
• Publication Date: 2023-11-30T13:24:27Z
• Authors: Xin Ting Zheng; Melisa Wei Ning Leoi; Yong Yu; Sherwin Chong Li Tan; Naeem Nadzri; Wei Peng Goh; Changyun Jiang; Xi Ping Ni; Pei Wang; Meng Zhao; Le Yang
• Keywords: Glucose oxidase; Bioelectronics

Abstract Wearable metabolite sensors are often limited by easily denaturable enzymes that only allow short‐duration monitoring. Although encapsulating in metal–organic frameworks (MOFs) shows promise of long‐term enzyme protection, it is typically accompanied significantly decreased activity due to increased diffusion barrier, steric hindrance for enzyme‐substrate binding, and poor enzyme‐electronic interface. Herein, the co‐encapsulation ultrasmall arginine‐derived carbon dots (Argdot) into a mesoporous Zeolitic Imidazolate Framework‐8 (mZIF‐8) matrix enhancement effect Argdot on stability activity, which consequently improves electrochemical sensor's sensitivity investigated. Specifically, glucose oxidase (GOx)‐Argdot@mZIF‐8 nanocomposite consistently exhibits 40% higher compared control GOx@mZIF‐8, an improvement similarly demonstrated with another model lactate (LOx). Furthermore, GOx‐Argdot@mZIF‐8 displays excellent stability, retaining 100% initial over 30 days repeated testing at 37 °C. A touch‐based sensor prototype as reusable monitor finger‐tip sweat levels one month room temperature. This encapsulation strategy not useful developing monitoring capability, but also promising expand industry use under harsh conditions.