Conductive PANi/PEGDA Macroporous Hydrogels For Nerve Regeneration

• DOI: 10.1002/adhm.201200152
• Publication Date: 2012-11-26T19:25:55Z
• Authors: Vincenzo Guarino; Marco Antonio Alv...; Anna Borriello; Teresa Napolitano; Luigi Ambrosio
• Keywords: Neurons; Conductive polymers; PANi; Aniline Compounds; Cell Survival; Electric Conductivity; Biocompatible Materials; Electric Stimulation Therapy; Hydrogels; 02 engineering and technology; Cell Line; Nerve Regeneration; Polyethylene Glycols; Nerve regeneration; Materials Testing; Humans; Methacrylates; Hybrid materials; 0210 nano-technology; Macroporous materials

Only recently polymers with intrinsic conductive properties have been studied in relation to their incorporation into bioactive scaffolds for use tissue engineering. The reason this interest is that such could electrically stimulate cells and thus regulate specific cellular activities, by means influence the process of regeneration those tissues respond electrical impulses. In our work, macroporous hydrogels are developed controlled pore morphology enable sufficient cell signaling supply events inherent nerve regeneration. A hybrid material has prepared situ precipitation polyaniline (PANi) polyethyleneglycol diacrylate (PEGDA) solution, followed crosslinking via UV irradiation. porous architecture, characterized macropores from 136 μm 158 size, achieved sodium chloride particle leaching. we demonstrate PANi synthesis hydrogel combine design materials suitable behaviour. presence evidently increased conductivity (1.1 ± 0.5) × 10(-3) mS/cm a content 3wt%. hydrophilic nature also enhanced water retention/proton more than one order magnitude. vitro studies confirmed 3 wt% improve biological response PC12 hMSC cells. Hybrid PANi/PEGDA supplement new functionalities terms morphological properties, both which essential prerequisites drive regenerative processes.