Angiogenic Type I Collagen Extracellular Matrix Integrated with Recombinant Bacteriophages Displaying Vascular Endothelial Growth Factors

• DOI: 10.1002/adhm.201500534
• Publication Date: 2015-12-08T16:41:22Z
• Authors: Junghyo Yoon; Nuriye Korkmaz Zi...; Hyun‐Ji Park; Sewoon Han; Kyung Hoon Hwang; Jisoo Shin; Seung‐Woo Cho; Chang‐Hoon Nam; Seok Chung
• Keywords: 0301 basic medicine; 570; HYDROGELS; Nanofibers; Fluorescent Antibody Technique; Neovascularization, Physiologic; Enzyme-Linked Immunosorbent Assay; 612; Protein Engineering; Collagen Type I; 03 medical and health sciences; Growth Factors; Animals; Humans; Bacteriophages; PHAGE DISPLAY; Recombination, Genetic; Mice, Inbred BALB C; Vascular Endothelial Growth Factors; SUPRAMOLECULAR STRUCTURES; VEGF; Extracellular Matrix; Rats; Microscopy, Fluorescence; CELLS; THERAPEUTIC ANGIOGENESIS; Angiogenesis; Collagen; REGENERATIVE MEDICINE; Cell Surface Display Techniques

Here, a growth‐factor‐integrated natural extracellular matrix of type I collagen is presented that induces angiogenesis. The developed matrix adapts type I collagen nanofibers integrated with synthetic colloidal particles of recombinant bacteriophages that display vascular endothelial growth factor (VEGF). The integration is achieved during or after gelation of the type I collagen and the matrix enables spatial delivery of VEGF into a desired region. Endothelial cells that contact the VEGF are found to invade into the matrix to form tube‐like structures both in vitro and in vivo, proving the angiogenic potential of the matrix.