Analysis of Osteoclastogenesis/Osteoblastogenesis on Nanotopographical Titania Surfaces

• DOI: 10.1002/adhm.201500664
• Publication Date: 2016-02-18T22:24:05Z
• Authors: Robert K. Silverwood; Paul G. Fairhurst; Terje Sjöström; Findlay Welsh; Yuxin Sun; Gang Li; Bin Yu; Peter S. Young; Bo Su; Robert M. D. Meek; Matthew J. Dalby; Penelope M. Tsimb...
• Keywords: Nanopillar; Nanotopography

A focus of orthopedic research is to improve osteointegration and outcomes joint replacement. Material surface topography has been shown alter cell adhesion, proliferation, growth. The use nanotopographical features promote adhesion bone formation hoped clinical outcomes. Use block‐copolymer self‐assembled nanopatterns allows nanopillars form via templated anodization with control over height order, which be cellular importance. This project assesses the outcome a human marrow‐derived co‐culture adherent osteoprogenitors osteoclast progenitors on polished titania patterned 15 nm nanopillars, fabricated by technique. Substrate implantation in rabbit femurs performed confirm vivo bone/implant integration. Quantitative qualitative results demonstrate increased osteogenesis nanopillar substrate scanning electron microscopy, histochemical staining, real‐time quantitative reverse‐transcription polymerase chain reaction analysis performed. Osteoblast/osteoclast shows an increase osteoblastogenesis‐related gene expression reduction osteoclastogenesis. Supporting this vitro finding, substrates femora indicates implant/bone contact ≈20%. These favorable osteogenic characteristics potential