Epidermal Growth Factor–Releasing Radially Aligned Electrospun Nanofibrous Patches for the Regeneration of Chronic Tympanic Membrane Perforations

• DOI: 10.1002/adhm.201801160
• Publication Date: 2018-12-12T08:45:58Z
• Authors: Hoon Seonwoo; Beomyong Shin; Kyoung‐Je Jang; Myungchul Lee; Oak‐Sung Choo; Sang‐Bae Park; Yeong Cheol Kim; Mi‐Jin Choi; Jangho Kim; Pankaj Garg; Jeong Hun Jang; Yun‐Hoon Choung; Jong Hoon Chung
• Keywords: Epidermal Growth Factor; Tympanic Membrane Perforation; Cell Survival; Guided Tissue Regeneration; Nanofibers; Rats, Sprague-Dawley; Drug Liberation; 03 medical and health sciences; 0302 clinical medicine; Gene Expression Regulation; Spectroscopy, Fourier Transform Infrared; Microscopy, Electron, Scanning; Animals; Female

Abstract Chronic tympanic membrane (TM) perforations can cause otorrhea. To date, various types of tissue engineering techniques have been applied for the regeneration chronic TM perforations. However, application nanofibers with radially aligned nanostructures and simultaneous release growth factors never in Here, epidermal factor (EGF)–releasing nanofibrous patches (ERA‐NFPs) are developed perforated TMs. First, radial alignments presence EGF ERA‐NFPs analyzed. is confirmed to be released from until 8 weeks. In an vitro study, cell viability assay, immunocytochemistry, wound‐healing assay indicate rational enhancement healing by combination release. The effect on cells revealed quantitative real‐time polymerase chain reaction. An vivo animal study shows that effectively stimulates TMs healed show histological properties similar those normal These results may efficient platform perforations, laying foundation nonsurgical treatments otitis media.