Preparation of Decellularized Triphasic Hierarchical Bone‐Fibrocartilage‐Tendon Composite Extracellular Matrix for Enthesis Regeneration

• DOI: 10.1002/adhm.201900831
• Publication Date: 2019-08-29T06:34:13Z
• Authors: Mingzhen Su; Qi Zhang; Yiwei Zhu; Shengyu Wang; Jiawen Lv; Jiaan Sun; Pengchen Qiu; Shunwu Fan; Keke Jin; Lei Chen; Xianfeng Lin
• Keywords: Enthesis; Fibrocartilage; Decellularization

Abstract Tendon to bone (enthesis) rupture, which may cause disability and persistent pain, shows high rate of re‐rupture after surgical repair. or enthesis scaffolds have been widely studied, but few these materials can recapitulate the tissue continuity. Thus, this study is conducted prepare a triphasic decellularized bone‐fibrocartilage‐tendon (D‐BFT) composite scaffold. The D‐BFT scaffold developed using combination physical, chemical, enzymatic treatments liquid nitrogen, Triton‐X 100, sodium‐dodecyl sulfate, DNase I, effectively removes cell components while preserving biological microstructure. Moreover, mechanical properties are highly preserved similar those human Achilles tendon. Additionally, in vitro, mesenchymal stem cells (MSCs) adhered, proliferated, infiltrated into scaffold, MSC differentiation confirmed by up‐regulation osteogenic‐related tenogenic‐related genes. repair outcomes explored applying model femur‐tibia defects vivo, good results. promising graft for regeneration.