Biomineralization Directed by Prenucleated Calcium and Phosphorus Nanoclusters Improving Mechanical Properties and Osteogenic Potential of Antheraea pernyi Silk Fibroin‐Based Artificial Periosteum

• DOI: 10.1002/adhm.202001695
• Publication Date: 2021-03-15T10:09:49Z
• Authors: Yajun Shuai; Huan Lu; Ruyin Lv; Jie Wang; Quan Wan; Chuanbin Mao; Mingying Yang
• Keywords: Fibroin; Nanoclusters; Antheraea pernyi

Abstract The use of biomacromolecules as templates to control the nucleation and growth hydroxyapatite crystals prepare bioactive materials is a valuable approach in bone tissue engineering. Here, an artificial periosteum prepared by biomineralizing Antheraea pernyi fibroin ( AF ) membrane with prenucleated nanoclusters, which can promote osteogenic differentiation mesenchymal stem cells (MSCs) induce formation matrix protein vivo. To achieve this, biologically inspired calcium phosphorus nanocluster mineralization system designed nucleate generate on surface membrane. This biomineralization process provides membranes improved elastic modulus tensile strength. Subsequently, cell viability assay, hemolysis test, H&E staining show that mineralized MAF has good cytocompatibility, hemocompatibility, histocompatibility vitro Additionally, significantly MSCs absence inducer vitro. Experiments vivo demonstrate bone‐related proteins are highly expressed groups or without seeded. Therefore, bioinspired nanoclusters based biomineralized promising strategy field