Sulfated Alginate Hydrogels Prolong the Therapeutic Potential of MSC Spheroids by Sequestering the Secretome

• DOI: 10.1002/adhm.202101048
• Publication Date: 2021-09-06T06:44:45Z
• Authors: Marissa Gionet‐Go...; Alena Casella; Daphne Diloretto; Clara Ginnell; Katherine H. Griffin; Anne Bigot; J. Kent Leach
• Keywords: Spheroid

Abstract Cell‐based approaches to tissue repair suffer from rapid cell death upon implantation, limiting the window for therapeutic intervention. Despite robust lineage‐specific differentiation potential in vitro, function of transplanted mesenchymal stromal cells (MSCs) vivo is largely attributed their potent secretome comprising a variety growth factors (GFs). Furthermore, GF secretion markedly increased when MSCs are formed into spheroids. Native GFs sequestered within extracellular matrix (ECM) via sulfated glycosaminoglycans, increasing potency signaling compared unbound form. To address critical need prolong efficacy cells, alginate hydrogels modified with sulfate groups sequester endogenous heparin‐binding secreted by MSC The influence crosslinking method and modification assessed on mechanical properties, degradation rate, degree modification. Sulfated mixture MSC‐secreted biomolecules, thereby prolonging effect spheroids regeneration. longer durations retain bioactivity regulate endothelial tubulogenesis myoblast infiltration. This platform has benefit serve as valuable tool investigating sequestration.