4D Printing of Personalized‐Tunable Biomimetic Periosteum with Anisotropic Microstructure for Accelerated Vascularization and Bone Healing

• DOI: 10.1002/adhm.202202868
• Publication Date: 2023-05-12T13:19:30Z
• Authors: Chao Liu; Yiting Lou; Zheyuan Sun; Haiying Ma; Mouyuan Sun; Shengjie Li; Dongqi You; Junjie Wu; Binbin Ying; Wanghui Ding; Mengfei Yu; Huiming Wang
• Keywords: Periosteum

An ideal biomimetic periosteum is expected to wrap various bone surfaces orchestrate an optimal microenvironment for regeneration, including facilitating local vascularization, recruiting osteoblasts, and mineralizing the extracellular matrix (ECM). To mimic role of natural in promoting repair, a 4D printing technique inlay aligned cell sheets on shape-shifting hydrogel used, containing biophysical signals spatially adjustable physical properties, first time. The outer layer endows with ability digitally coordinate its 3D geometry match specific macroscopic shape maintain healing microenvironment. inner human mesenchymal stem cells (hMSCs) not only promotes migration angiogenesis co-cultured but also exhibits excellent osteogenic differentiation properties. In vivo experiments show that apart from morphing preset shapes as barriers, can actively facilitate early-stage osteogenesis. Altogether, this present work provides novel route construct personalized anisotropic microstructure by introducing tunable reconstruction strategy be extended repair sophisticated defects.