3D Bioprinting of Neurovascular Tissue Modeling with Collagen‐Based Low‐Viscosity Composites

• DOI: 10.1002/adhm.202300004
• Publication Date: 2023-06-02T13:04:28Z
• Authors: Sen Wang; Luge Bai; Xiaoxuan Hu; Siqi Yao; Zhiyan Hao; JiaJia Zhou; Xiao Li; Haixia Lu; Jiankang He; Ling Wang; Dichen Li
• Keywords: Neurovascular bundle; 3D bioprinting; Biomanufacturing

In vitro neurovascular unit (NVU) models are valuable for investigating brain functions and developing drugs. However, it remains challenging to recapitulate the native architectural features ultra-soft extracellular matrix (ECM) properties of natural NVU. Cell-laden bioprinting is promising prepare complex living tissues, but hard balance fidelity cell growth. This study proposes a novel two-stage methodology biomanufacturing functional 3D constructs in with low modulus ECM. At shaping stage, low-viscosity alginate/collagen printed through an embedded approach; at culturing alginate removed targeted lysing. The rapid crosslinking provide printing resolution ≈10 µm, lysis processing can decrease hydrogels' ≈1 kPa adjust porosity microstructure, providing cells environment closing A hollow coaxial model fabricated, which endothelial has expressed tight junction proteins shown selective permeability, astrocytes outside layer found spread out branches directly interact cells. present offers modeling method better understanding NVU function screening neuro-drugs.