Oxidative Stimuli‐Responsive “Pollen‐Like” Exosomes from Silver Nanoflowers Remodeling Diabetic Wound Microenvironment for Accelerating Wound Healing

• DOI: 10.1002/adhm.202300456
• Publication Date: 2023-05-13T11:25:05Z
• Authors: Yahong Chen; Muhammad Rizwan Y...; Gang He; Zhiwei Zheng; Yun Wang; Ke Xue; Jian Sun; Kai Liu; Peng Huang; Xiansong Wang
• Keywords: Mice; Wound Healing; Oxidative Stress; Silver; Diabetes Mellitus; Animals; Collagen; Exosomes

The hostile oxidative wound microenvironment, defective angiogenesis, and uncontrolled release of therapeutic factors are major challenges in improving the diabetic healing. Herein, adipose-derived-stem-cell-derived exosomes (Exos) first loaded into Ag@bovine serum albumin (BSA) nanoflowers (Exos-Ag@BSA NFs) to form a protective "pollen-flower" delivery structure, which further encapsulated injectable collagen (Col) hydrogel NFs/Col) for concurrent remodeling microenvironment precise Exos. Exos-Ag@BSA NFs can selectively dissociate an triggers sustained Ag ions (Ag+ ) cascades controllable "pollen-like" Exos at target site, thus protecting from denaturation. Such wound-microenvironment-activated property Ag+ effectively eliminates bacteria promotes apoptosis impaired cells, resulting improved regenerative microenvironment. Additionally, NFs/Col markedly accelerates healing regeneration vivo murine silicone-splinted excisional model by promoting blood perfusion, tissue granulation, deposition, neovascularization, re-epithelization. It is anticipated that this work will inspire development more delicate disease-specific systems clinical management.