Embedded Bioprinting of Breast Tumor Cells and Organoids Using Low‐Concentration Collagen‐Based Bioinks

• DOI: 10.1002/adhm.202300905
• Publication Date: 2023-07-09T02:30:01Z
• Authors: Wen Shi; Sameer Mirza; Mitchell Kuss; Bo Liu; Andrew Hartin; Shibiao Wan; Yunfan Kong; Bhopal Mohapatra; Mena Krishnan; Hamid Band; Vimla Band; Bin Duan
• Keywords: 3D bioprinting; Organoid; Fibroin

Bioinks for 3D bioprinting of tumor models should not only meet printability requirements but also accurately maintain and support phenotypes surrounding cells to recapitulate key hallmarks. Collagen is a major extracellular matrix protein solid tumors, low viscosity collagen solution has made bioprinted cancer challenging. This work produces embedded, breast organoid using low-concentration I based bioinks. The biocompatible physically crosslinked silk fibroin hydrogel used generate the bath embedded printing. composition bioink optimized with thermoresponsive hyaluronic acid-based polymer both noninvasive epithelial invasive cells, as well cancer-associated fibroblasts. Mouse organoids are mimic in vivo morphology. A vascularized model created similar strategy, significantly enhanced vasculature formation under hypoxia. study shows great potential utilizing collagen-based advancing understanding cell biology facilitating drug discovery research.