3D Elastomeric Stent Functionalized with Antioxidative and Perivascular Tissue Regenerative Activities Ameliorated PVT Deprivation‐Induced Vein Graft Failure

• DOI: 10.1002/adhm.202301247
• Publication Date: 2023-07-13T18:19:53Z
• Authors: Yinggang Wang; Mingdi Fu; Weiwei Xiao; Yajing Zhao; Pingping Yuan; Xinchi Zhang; Wei Wu
• Keywords: Regenerative Medicine

Clinically, arterial injuries are always accompanied with perivascular tissue damage, which may contribute to high failure rate of vein grafts due intimal hyperplasia and acute thrombosis. In this study, a "perivascular (PVT) deprivation" animal model is constructed mimic clinical scenarios identify the contribution PVT success grafts. Proteomics analysis suggests that depriving exacerbate reactive oxygen species (ROS)-induced endothelial apoptosis by up-regulating inflammation response oxidative stress. Locally administering metformin on through 3D-printed external stent (PGS-PCL) shows antioxidative anti-inflammatory properties protect cells from ROS invasion, thereafter decreasing Moreover, induce rapid regeneration adipose in recipient regions, improves patency inhibiting hyperplasia. Proteomics, western blot, vitro blocking tests reveal resists AMPK/mTOR NFκB signaling pathways. To conclude, deprivation exacerbates inflammatory stress bridging circulation. Metformin-loaded ameliorates "PVT damage" related graft failure, enhances resisting regenerating PVAT, offering promising avenue improve clinic.