Oxidized Low‐Density Lipoprotein (Ox‐LDL)‐Triggered Double‐Lock Probe for Spatiotemporal Lipoprotein Oxidation and Atherosclerotic Plaque Imaging

• DOI: 10.1002/adhm.202301595
• Publication Date: 2023-08-09T23:03:31Z
• Authors: Xu Zhi; Yufen Sun; Fangjian Cai; Sisi Wang; Hu Gao; Fan Wu; Lei Zhang; Zhen Shen
• Keywords: Carbazole; Low-density lipoprotein; Chromophore

Low-density lipoprotein (LDL), especially oxidative modified LDL (Ox-LDL), is the key risk factor for plaque accumulation and development of cardiovascular disease. Herein, a highly specific Ox-LDL-triggered fluorogenic-colorimetric probe Pro-P1 developed visualizing oxidation aggregation progress lipoproteins plaque. A series green fluorescent protein chromophores with donor-acceptor structures, containing carbazole as an electron donor various substituents including pyridine-vinyl (P1), phenol-vinyl (P2), N, N-dimethylaniline-vinyl (P3), thiophene-vinyl (P4), have been synthesized evaluated. Emission spectroscopy theoretical calculations P1-P4 indicate that P1 shows enhanced fluorescence (λem = 560 nm) by inhibiting its twisted intramolecular charge transfer in presence Ox-LDL. This feature allows selection sensitive to directly visualize ferroptosis Cu2+ -mediated via situ formation fluorophore-bound Ox-LDL living cells. The red-emissive 660 prepared borate protection P1, which can be cleaved into under high expression HOCl condition at lesion site, resulting emission. area size clear boundaries delineated colorimetric imaging lifetime precise differentiation.