NIR‐II Cyazulene Fluorophores with Adjustable Cycloalkenes and Spectroscopic Properties for Imaging Applications

• DOI: 10.1002/adhm.202404996
• Publication Date: 2025-02-16T16:30:16Z
• Authors: Wenjie Gao; Diankai Liu; He Li; Zixu He; Ya Liu; Xiaohua Li; Huimin Ma; Wen Shi
• Keywords: Azulene; Fluorescence-lifetime imaging microscopy; Spectral Properties

Abstract NIR‐II fluorescence imaging has shown broad application prospects in life science because of its deeper tissue penetration and higher spatiotemporal resolution. Developing fluorophores with special spectroscopic properties analytical functions become a cutting‐edge but challenging topic this field. Herein, series azulene‐based (called cyazulenes) adjustable cycloalkenes for applications is reported. Cyazulenes (CA965, CA985, CA1025) are constructed via the convenient coupling azulene derivatives. They exhibit different H‐aggregation J‐aggregation. Particularly noteworthy that CA985 can form significant J‐aggregates strong sharp emission biological media, be used vivo blood vessels fine structures collecting lymphatics mice. Moreover, displays passive bone‐targeted capacity, thereby enabling bone imaging. This study demonstrates chemical impact varying on aggregate formation spectral cyazulenes, which will advance fluorophores.