A Self‐Assembled Nanoreactor for Realizing Antibacterial Photodynamic/Gas Therapy and Promoting Wound Healing

• DOI: 10.1002/adhm.202500487
• Publication Date: 2025-04-28T05:25:34Z
• Authors: Chenyang Jia; Ling‐Yi Li; Qiu‐Yi Duan; Xiaoyang Liu; Xiao‐Yu Zhu; Ke‐Fei Xu; Chengcheng Li; Zi‐Xi Wang; Fu‐Gen Wu
• Keywords: Nanoreactor; Protoporphyrin IX

Abstract Among various treatments employed to solve the global problem of bacterial infection, photodynamic therapy (PDT) is recognized as a method with great potential inactivate wide range bacteria without development drug resistance. However, many commonly used photosensitizers (PSs) have disadvantages poor water‐solubility and toxicity, which limits their clinical application. Additionally, nitric oxide (NO) has unique advantages in antibacterial due its small molecular weight. Herein, protoporphyrin IX (PpIX), L‐arginine (L‐Arg), glycol chitosan (GC) are construct self‐assembled cationic Arg‐GC‐PpIX nanoreactor for efficient inactivation under white light illumination. The excellent water dispersity stability can rapidly bind through electrostatic interaction produce local singlet oxygen ( 1 O 2 )/NO irradiation, leading high efficiency toward both Gram‐negative Gram‐positive bacteria. Besides, these NPs also possess desirable antibiofilm ability. Finally, Arg‐GC‐PpIX@Gel obtained loading into sodium alginate (SA)/Ca 2+ hydrogel shows satisfactory ability promote infected wound healing when combined irradiation. Therefore, rationally designed light‐triggered /NO release promising agent achieving effective PDT/NO gas therapy.