Synthesis and Assembly of Click‐Nucleic‐Acid‐Containing PEG–PLGA Nanoparticles for DNA Delivery

• DOI: 10.1002/adma.201700743
• Publication Date: 2017-04-11T12:18:38Z
• Authors: Albert Harguindey; Dylan W. Domaille; Benjamin D. Fairb...; Justine Wagner; Christopher N. Bo...; Jennifer N. Cha
• Keywords: PLGA

Co-delivery of both chemotherapy drugs and siRNA from a single delivery vehicle can have significant impact on cancer therapy due to the potential for overcoming issues such as drug resistance. However, inherent chemical differences between charged nucleic acids hydrophobic hindered entrapment components within carrier. While poly(ethylene glycol)-block-poly(lactic-co-glycolic acid) (PEG-PLGA) copolymers been used successfully targeted drugs, loading DNA or RNA has poor. It is demonstrated that amounts be encapsulated PLGA-containing nanoparticles through use new synthetic analog, click (CNAs). First, triblock PEG-CNA-PLGA are synthesized then formulated into polymer oil-in-water emulsions. The CNA-containing particles show high encapsulation complementary CNA sequence, whereas PEG-PLGA alone shows minimal loading, non-complementary strands do not get nanoparticles. Furthermore, dye pyrene co-loaded with lastly, complex, larger sequence contains an overhang also encapsulated, demonstrating utility carriers agents gene silencers.