Aqueous Two‐Phase Emulsion Bioink‐Enabled 3D Bioprinting of Porous Hydrogels

• DOI: 10.1002/adma.201805460
• Publication Date: 2018-10-22T05:46:25Z
• Authors: Guo‐Liang Ying; Nan Jiang; Sushila Maharjan; Yi‐Xia Yin; Rong‐Rong Chai; Xia Cao; Jing‐Zhou Yang; Amir K. Miri; Shabir Hassan; Yu Shrike Zhang
• Keywords: 3D bioprinting; Aqueous two-phase system

3D bioprinting technology provides programmable and customizable platforms to engineer cell-laden constructs mimicking human tissues for a wide range of biomedical applications. However, the encapsulated cells are often restricted in spreading proliferation by dense biomaterial networks from gelation bioinks. Herein, cell-benign approach is reported directly bioprint porous-structured hydrogel using an aqueous two-phase emulsion bioink. The bioink, which contains two immiscible phases cell/gelatin methacryloyl (GelMA) mixture poly(ethylene oxide) (PEO), photocrosslinked fabricate predesigned extrusion or digital micromirror device-based stereolithographic bioprinting. porous structure 3D-bioprinted construct formed subsequently removing PEO phase GelMA hydrogel. Three different cell types (human hepatocellular carcinoma cells, umbilical vein endothelial NIH/3T3 mouse embryonic fibroblasts) within patterns show enhanced viability, spreading, compared standard (i.e., nonporous) constructs. strategy believed provide robust versatile platform tissue their models variety applications engineering, regenerative medicine, drug development, personalized therapeutics.