Adaptive Liquid Interfacially Assembled Protein Nanosheets for Guiding Mesenchymal Stem Cell Fate

• DOI: 10.1002/adma.201905942
• Publication Date: 2019-12-09T08:01:41Z
• Authors: Xiaofang Jia; Kosuke Minami; Koichiro Uto; Alice Chinghsuan ...; Jonathan P. Hill; Jun Nakanishi; Katsuhiko Ariga
• Keywords: Neurons; Extracellular Matrix Proteins; Fluorocarbons; Surface Properties; Biocompatible Materials; Cell Differentiation; Mesenchymal Stem Cells; 01 natural sciences; Extracellular Matrix; Fibronectins; Nanostructures; 0104 chemical sciences; Focal Adhesion Protein-Tyrosine Kinases; Cell Adhesion; Humans; Cells, Cultured

There is a growing interest in the development of dynamic adaptive biomaterials for regulation cellular functions. However, existing materials are limited to two-state switching presentation and removal cell-adhesive bioactive motifs that cannot emulate native extracellular matrix (ECM) vivo with continuously adjustable characteristics. Here, tunable composed protein monolayer assembled at liquid-liquid interface demonstrated, which adapt dynamically cell traction forces. An ultrastructure transition from hierarchical fiber occurs through interfacial jamming. Elongated fibronectin fibers promote formation elongated focal adhesion structures, increase kinase activation, enhance neuronal differentiation stem cells. Cell force results spatial rearrangement ECM proteins, feeds back alter fate. The reported biomimetic liquid enables control behavior has potential translational applications.