Expanding Embedded 3D Bioprinting Capability for Engineering Complex Organs with Freeform Vascular Networks

• DOI: 10.1002/adma.202205082
• Publication Date: 2023-02-16T21:26:47Z
• Authors: Yongcong Fang; Yihan Guo; Bingyan Wu; Zibo Liu; Min Ye; Yuanyuan Xu; Mengke Ji; Li Chen; Bingchuan Lu; Kaiji Nie; Zixuan Wang; Jianbin Luo; Ting Zhang; Wei Sun; Zhuo Xiong
• Keywords: Biofabrication; 3D bioprinting; 3d printed; Vascular network

Creating functional tissues and organs in vitro on demand is a major goal biofabrication, but the ability to replicate external geometry of specific their internal structures such as blood vessels simultaneously remains one greatest impediments. Here, this limitation addressed by developing generalizable bioprinting strategy sequential printing reversible ink template (SPIRIT). It demonstrated that microgel-based biphasic (MB) bioink can be used both an excellent suspension medium supports embedded 3D due its shear-thinning self-healing behavior. When encapsulating human-induced pluripotent stem cells, MB printed generate cardiac organoids extensive cell proliferation differentiation. By incorporating bioink, SPIRIT enables effective ventricle model with perfusable vascular network, which not possible fabricate using extant strategies. This technique offers unparalleled capability complex organ structure faster manner, will accelerate biofabrication therapeutic applications tissue constructs.